Influence of two-phase system composition on biocatalytic properties of β-galactosidase preparations

被引：11

作者：

Bednarski, W. ^{[1
]}

Kulikowska, A. ^{[1
]}

机构：

[1] Univ Warmia & Mazury Olsztyn, Chair Food Biotechnol, PL-10718 Olsztyn, Poland

来源：

CHEMICAL PAPERS | 2007年 / 61卷 / 05期

关键词：

beta-galactosidase; immobilized biocatalyst; organic solvents; lactose; galactooligosaccharides;

D O I：

10.2478/s11696-007-0049-9

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A comparison was made between the catalytic properties of beta-galactosidase from Penicillium canescens and Aspergillus oryzae using lactose in two-phase systems of an organic solvent and water. It was shown that the ability of beta-galactosidase preparations to synthesize galactooligosaccharides depends on the type of the solvent used (cyclohexane, hexane, toluene) and on its proportion in the two-phase system. The use of cyclohexane in the reaction medium stabilized the activity of beta-galactosidase from P. canescens which was immobilized on tosylated cotton cloth, and enabled its multiple use in galactooligosaccharides synthesis.

引用

页码：364 / 369

页数：6

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