An In vitro Comparative Evaluation of Microleakage of ACP Containing Pit and Fissure Sealant and Moisture Tolerant Pit and Fissure Sealant

Background: Pits and fissure sealants are widely used to prevent caries in children. Microleakage is one of the most crucial factors resulting in sealant compromise. Factors pertaining to microleakage like, pre-treatment of occlusal surfaces, moisture control, bonding systems, and flowability of the sealant, will determine the longevity of the treatment. In vitro microleakage studies are useful in predicting the marginal sealing ability of pit and fissure sealants. Aim: This in vitro study was undertaken to evaluate and compare the microleakage of ACP (Aegis (R)) containing pit and fissure sealant and moisture tolerant pit and fissure sealant (Embrace (TM) Wetbond (TM)). Study Design: In vitro study. Place and Duration of Study: Department of Pedodontics and Preventive Dentistry, Dr. D.Y. Patil Dental College and Hospital, Pune. between June 2020 and February 2021. Methodology: 26 permanent non-carious premolars extracted for orthodontic treatment were used. The sealants used for the analysis of microleakage were Aegis (R) and Embrace (TM) Wetbond (TM) pit and fissure sealants. The premolars were divided into two groups of 13 each. After sealant application, they were immersed in methylene blue dye. The teeth were subjected to thermocycling for 24 hours, after which The teeth were sectioned buccolingually and dye penetration was studied under a stereomicroscope (magnification 10X). Results: Both the groups were studied and statistically evaluated using Mann-Whitney U test. Aegis (R) showed higher microleakage in (46.2%) than Embrace (38%), though the results were not statistically significant. Conclusion: Both the materials showed microleakage, more In-vivo studies with longer follow- up periods are needed to evaluate and compare the clinical success of these pit & fissure sealants.