5-Hydroxytryptamine 5HT2C Receptors Form a Protein Complex with N-Methyl-D-aspartate GluN2A Subunits and Activate Phosphorylation of Src Protein to Modulate Motoneuronal Depolarization

被引：20
作者：
Bigford, Gregory E. [3 ,4 ]
Chaudhry, Nauman S. [3 ]
Keane, Robert W. [1 ,3 ,4 ]
Holohean, Alice M. [2 ,4 ]
机构：
[1] Univ Miami, Miller Sch Med, Dept Physiol & Biophys, Miami, FL 33101 USA
[2] Univ Miami, Miller Sch Med, Dept Neurol, Miami, FL 33101 USA
[3] Univ Miami, Miller Sch Med, Miami Project Cure Paralysis, Miami, FL 33101 USA
[4] Miami Vet Affairs Med Ctr, Res Serv, Miami, FL 33125 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 14期
关键词：
LUMBAR SPINAL-CORD; MESSENGER-RNA EXPRESSION; GATED ION CHANNELS; NMDA RECEPTORS; 5-HT2C RECEPTORS; FROG MOTONEURONS; CONSTITUTIVE ACTIVITY; TYROSINE KINASE; AMINO-ACID; GLUTAMATE RECEPTORS;
D O I：
10.1074/jbc.M111.277806
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: 5-Hydroxytryptamine (5HT) modulates N-methyl-D-aspartate ( NMDA) depolarization. Results: 5HT2C co-immunoprecipitates with GluN2A and enhances NMDA motoneuronal depolarization through phosphorylation of Src(Tyr-416). Conclusion: 5HT modulates NMDA through Src phosphorylation in a molecular complex that is localized in the processes of spinal neurons. Significance: 5HT2C modulation of NMDA excitation is coordinated by a molecular complex.
引用
收藏
页码：11049 / 11059
页数：11
相关论文
共 82 条
  • [1] NMDA receptor regulation by Src kinase signalling in excitatory synaptic transmission and plasticity
    Ali, DW
    Salter, MW
    [J]. CURRENT OPINION IN NEUROBIOLOGY, 2001, 11 (03) : 336 - 342
  • [2] Alvarez FJ, 1998, J COMP NEUROL, V393, P69
  • [3] Banes A, 1999, J PHARMACOL EXP THER, V291, P1179
  • [4] Barnes N. M., 1999, NEUROPHARMACOLOGY, V524, P171
  • [5] A review of central 5-HT receptors and their function
    Barnes, NM
    Sharp, T
    [J]. NEUROPHARMACOLOGY, 1999, 38 (08) : 1083 - 1152
  • [6] 5-Hydroxytryptamine4 receptor activation of the extracellular signal-regulated kinase pathway depends on src activation but not on G protein or β-arrestin signaling
    Barthet, Gael
    Framery, Berenice
    Gaven, Florence
    Pellissier, Lucie
    Reiter, Eric
    Claeysen, Sylvie
    Bockaert, Joel
    Dumuis, Aline
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (06) : 1979 - 1991
  • [7] Serotonin-induced inhibition of locomotor rhythm of the rat isolated spinal cord is mediated by the 5-HT1 receptor class
    Beato, M
    Nistri, A
    [J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1998, 265 (1410) : 2073 - 2080
  • [8] The serotonin 5-HT2A and 5-HT2C receptors interact with specific sets of PDZ proteins
    Bécamel, C
    Gavarini, S
    Chanrion, B
    Alonso, G
    Galéotti, N
    Dumuis, A
    Bockaert, J
    Marin, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (19) : 20257 - 20266
  • [9] Synaptic multiprotein complexes associated with 5-HT2C receptors:: a proteomic approach
    Bécamel, C
    Alonso, G
    Geléotti, N
    Demey, E
    Jouin, P
    Ullmer, C
    Dumuis, A
    Bockaert, J
    Marin, P
    [J]. EMBO JOURNAL, 2002, 21 (10) : 2332 - 2342
  • [10] Effector pathway-dependent relative efficacy at serotonin type 2A and 2C receptors: Evidence for agonist-directed trafficking of receptor stimulus
    Berg, KA
    Maayani, S
    Goldfarb, J
    Scaramellini, C
    Leff, P
    Clarke, WP
    [J]. MOLECULAR PHARMACOLOGY, 1998, 54 (01) : 94 - 104
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