Engineering of a light-gated potassium channel

被引：102

作者：

Cosentino, Cristian ^{[1
]}

Alberio, Laura ^{[1
]}

Gazzarrini, Sabrina ^{[1
]}

Aquila, Marco ^{[1
]}

Romano, Edoardo ^{[1
]}

Cermenati, Solei ^{[1
]}

Zuccolini, Paolo ^{[1
]}

Petersen, Jan ^{[2
]}

Beltrame, Monica ^{[1
]}

Van Etten, James L. ^{[3
,4
]}

Christie, John M. ^{[2
]}

Thiel, Gerhard ^{[5
]}

Moroni, Anna ^{[1
]}

机构：

[1] Univ Milan, Dept Biosci, I-20122 Milan, Italy

[2] Univ Glasgow, Inst Mol Cell & Syst Biol, Glasgow G12 8QQ, Lanark, Scotland

[3] Univ Nebraska Lincoln, Dept Plant Pathol, Lincoln, NE 68583 USA

[4] Univ Nebraska Lincoln, Nebraska Ctr Virol, Lincoln, NE 68583 USA

[5] Tech Univ Darmstadt, Membrane Biophys, Darmstadt, Germany

来源：

SCIENCE | 2015年 / 348卷 / 6235期

基金：

英国生物技术与生命科学研究理事会; 美国国家科学基金会;

关键词：

CHLORIDE CHANNEL; ION CHANNELS; K+ CHANNEL; CHANNELRHODOPSIN; DOMAIN; LOV;

D O I：

10.1126/science.aaa2787

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K+) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K+ channel 1 (BLINK1) engineered by fusing the plant LOV2-J alpha photosensory module to the small viral K+ channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K+ selectivity and high single-channel conductance, but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K+ equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.

引用

页码：707 / 710

页数：4

共 23 条

[1] Protein palmitoylation and subcellular trafficking [J].