De-Novo Assembly and Analysis of the Heterozygous Triploid Genome of the Wine Spoilage Yeast Dekkera bruxellensis AWRI1499

被引：100

作者：

Curtin, Chris D. ^{[1
]}

Borneman, Anthony R. ^{[1
]}

Chambers, Paul J. ^{[1
]}

Pretorius, Isak S. ^{[1
,2
]}

机构：

[1] Australian Wine Res Inst, Adelaide, SA, Australia

[2] Univ S Australia, Adelaide, SA 5001, Australia

来源：

PLOS ONE | 2012年 / 7卷 / 03期

关键词：

VINYLPHENOL REDUCTASE; HYDROXYCINNAMIC ACIDS; POPULATION-DYNAMICS; ALCOHOL PRODUCTION; WEB SERVER; SACCHAROMYCES; GENE; EVOLUTION; GROWTH; SSU1;

D O I：

10.1371/journal.pone.0033840

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.

引用

页数：10

共 51 条

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