Strongyloides venezuelensis:: The antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis

被引：23
作者：
Machado, Eleuza Rodrigues [1 ]
Faccioli, Lucia Helena [2 ]
Costa-Cruz, Julia Maria [3 ]
Lourenco, Elaine Vicente [4 ]
Roque-Barreira, Maria Cristina [4 ]
de Fatima Goncalves Pires, Maria do Rosario [3 ]
Ueta, Marlene Tiduko [1 ]
机构：
[1] Univ Estadual Campinas, Inst Biol, Dept Parasitol, BR-13083970 Campinas, SP, Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, Brazil
[3] Univ Fed Uberlandia, Inst Ciencias Biomed, Dept Microbiol Immunol & Parasitol, BR-38400 Uberlandia, MG, Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Imunol, Ribeirao Preto, Brazil
来源：
EXPERIMENTAL PARASITOLOGY | 2008年 / 119卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
Strongyloides venezuelensis; strongyloidiasis; diagnosis; antigen; antibodies; IFAT; ELISA; immunoblot;
D O I：
10.1016/j.exppara.2007.12.008
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of U were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite. (C) 2008 Elsevier Inc. All rights reserved.
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页码：7 / 14
页数：8
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