Microbe-focused glycan array screening platform

被引：71

作者：

Geissner, Andreas ^{[1
,2
,9
]}

Reinhardt, Anika ^{[1
,2
,10
]}

Rademacher, Christoph ^{[1
,2
]}

Johannssen, Timo ^{[1
,2
]}

Monteiro, Joao ^{[3
,4
]}

Lepenies, Bernd ^{[1
,2
,3
,4
]}

Thepaut, Michel ^{[5
,6
,7
]}

Fieschi, Franck ^{[5
,6
,7
]}

Mrazkova, Jana ^{[8
]}

Wimmerova, Michaela ^{[8
]}

Schuhmacher, Frank ^{[1
,2
]}

Goetze, Sebastian ^{[1
,2
,11
]}

Gruenstein, Dan ^{[1
,2
]}

Guo, Xiaoqiang ^{[1
,2
,12
]}

Hahm, Heung Sik ^{[1
,2
,13
]}

Kandasamy, Jeyakumar ^{[1
,14
]}

Leonori, Daniele ^{[1
,15
]}

Martin, Christopher E. ^{[1
,2
,16
]}

Parameswarappa, Sharavathi G. ^{[1
,17
]}

Pasari, Sandip ^{[1
,18
]}

Schlegel, Mark K. ^{[1
,19
]}

Tanaka, Hidenori ^{[1
,20
]}

Xiao, Guozhi ^{[1
,21
]}

Yang, You ^{[1
,22
]}

Pereira, Claney L. ^{[1
,17
]}

Anish, Chakkumkal ^{[1
,23
]}

Seeberger, Peter H. ^{[1
,2
]}

机构：

[1] Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14424 Potsdam, Germany

[2] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany

[3] Univ Vet Med Hannover, Immunol Unit, D-30559 Hannover, Germany

[4] Univ Vet Med Hannover, Res Ctr Emerging Infect & Zoonoses, D-30559 Hannover, Germany

[5] Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France

[6] CNRS, Inst Biol Struct, F-38044 Grenoble, France

[7] Commissariat Energie Atom & Energies Alternat, Inst Biol Struct, F-38044 Grenoble, France

[8] Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic

[9] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada

[10] Kyoto Univ, Dept Life Sci Frontiers, Ctr iPS Cell Res & Applicat, Kyoto 6068507, Japan

[11] Univ Koblenz Landau, Fac Nat & Environm Sci 7, D-76829 Landau, Germany

[12] Clariant Innovat Ctr, D-65929 Frankfurt, Germany

[13] Univ Virginia, Dept Chem, Charlottesville, VA 22904 USA

[14] Indian Inst Technol, Dept Chem, Varanasi 221005, Uttar Pradesh, India

[15] Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England

[16] Bachem AG, CH-4416 Bubendorf, Switzerland

[17] Vaxxilon Deutschland GmbH, D-12489 Berlin, Germany

[18] Indian Inst Sci Educ & Res, Pune 411008, Maharashtra, India

[19] Alnylam Pharmaceut, Cambridge, MA 02142 USA

[20] Kochi Univ, Ctr Sci Res, Oceanog Sect, Otsu 200, Nankoku, Kochi 7838503, Japan

[21] Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China

[22] East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

[23] Janssen Pharmaceut Johnson & Johnson, Bacterial Vaccines Discovery & Early Dev, NL-2333 CK Leiden, Netherlands

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2019年 / 116卷 / 06期

基金：

欧洲研究理事会; 欧盟地平线“2020”;

关键词：

glycan arrays; microbial antigens; immune receptors; antiglycan antibodies; bacterial lectins; C-TYPE LECTINS; BURKHOLDERIA-CENOCEPACIA LECTIN; DC-SIGN; CAPSULAR POLYSACCHARIDE; STRUCTURAL BASIS; MICROARRAY TECHNOLOGY; BACILLUS-ANTHRACIS; VACCINE CANDIDATE; FUNCTIONAL-ROLE; BINDING;

D O I：

10.1073/pnas.1800853116

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

引用

页码：1958 / 1967

页数：10

共 89 条

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