Analysis of cell cultures of Taxus wallichiana using direct analysis in real-time mass spectrometric technique

被引：18
|
作者：
Banerjee, Suchitra [1 ]
Madhusudanan, K. P. [2 ]
Khanuja, Suman P. S. [1 ]
Chattopadhyay, Sunil K. [1 ]
机构：
[1] Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India
[2] Cent Drug Res Inst, Sophisticated Analy Inst Facil, Lucknow 226001, Uttar Pradesh, India
来源：
BIOMEDICAL CHROMATOGRAPHY | 2008年 / 22卷 / 03期
关键词：
DART MS; analysis; calli; Taxus wallichiana;
D O I：
10.1002/bmc.919
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the calli of Taxus wallichiana. The intact callus samples were directly analyzed by holding them in the gap between the DART source and mass spectrometer for measurements. Five C-14 oxygenated taxoids were characterized from the analysis of the calli of the Taxus wallichiana almost instantaneously. The confirmation of the structures of the identified taxoids was made through their accurate molecular formula determinations. Copyright (c) 2007 John Wiley & Sons, Ltd.
引用
收藏
页码：250 / 253
页数：4
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