Multiplex detection of pathogen biomarkers in human blood, serum, and saliva using silicon photonic microring resonators

被引：7

作者：

Estrada, I. A. ^{[1
]}

Burlingame, R. W. ^{[1
]}

Wang, A. P. ^{[1
]}

Chawla, K. ^{[1
]}

Grove, T. ^{[1
]}

Wang, J. ^{[1
]}

Southern, S. O. ^{[2
]}

Iqbal, M. ^{[1
]}

Gunn, L. C. ^{[1
]}

Gleeson, M. A. ^{[1
]}

机构：

[1] Genalyte Inc, San Diego, CA 92121 USA

[2] Gaia Med Inst, La Jolla, CA 92037 USA

来源：

ADVANCES IN GLOBAL HEALTH THROUGH SENSING TECHNOLOGIES 2015 | 2015年 / 9490卷

关键词：

Antigen capture; assay development; multiplex; silicon photonics; ring resonance; LABEL-FREE; SENSORS; ARRAYS; SENSITIVITY; DENGUE;

D O I：

10.1117/12.2183403

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Genalyte has developed a multiplex silicon photonic chip diagnostics platform (Maverick (TM)) for rapid detection of up to 32 biological analytes from a drop of sample in just 10 to 20 minutes. The chips are manufactured with waveguides adjacent to ring resonators, and probed with a continuously variable wavelength laser. A shift in the resonant wavelength as mass binds above the ring resonators is measured and is directly proportional to the amount of bound macromolecules. We present here the ability to multiplex the detection of hemorrhagic fever antigens in whole blood, serum, and saliva in a 16 minute assay. Our proof of concept testing of a multiplex antigen-capture chip has the ability to detect Zaire Ebola (ZEBOV) recombinant soluble glycoprotein (rsGP), Marburg virus (MARV) Angola recombinant glycoprotein (rGP) and dengue nonstructural protein I (NS1). In parallel, detection of 2 malaria antigens has proven successful, but has yet to be incorporated into multiplex with the others. Each assay performs with sensitivity ranging from 1.6 ng/ml to 39 ng/ml depending on the antigen detected, and with minimal cross-reactivity.

引用

页数：14

共 28 条

[1] Label-free, single-molecule detection with optical microcavities [J].