Induction of mitotic crossing-over in diploid strains of Aspergillus nidulans using low-dose X-rays

被引：3

作者：

Stoll, L. B. ^{[3
]}

Cremonesi, F. C. A. ^{[4
]}

Pires, L. T. A. ^{[5
]}

Zucchi, T. D. ^{[1
,2
]}

Zucchi, T. M. A. D. ^{[1
,2
,6
]}

机构：

[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil

[2] Univ Sao Paulo, Ctr Pesquisas Biotecnol, BR-05508 Sao Paulo, Brazil

[3] Univ Franca, Fac Odontol, Franca, SP, Brazil

[4] Univ Sao Paulo, Fac Odontol, BR-14049 Ribeirao Preto, SP, Brazil

[5] Ctr Univ Moura Lacerda, Ribeirao Preto, SP, Brazil

[6] Univ Estado Sao Paulo, Dept Ciencias Biol & Biotecnol, Assis, SP, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2008年 / 7卷 / 02期

关键词：

low-dose X-rays; mitotic crossing-over; Aspergillus nidulans; genotoxicity tests; DNA damage; homozygotization index assay;

D O I：

10.4238/vol7-2gmr414

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a contribution towards detecting the genetic effects of low doses of genotoxic physical agents, this paper deals with the consequences of low-dose X-rays in the Aspergillus nidulans genome. The irradiation doses studied were those commonly used in dental clinics (1-5 cGy). Even very low doses promoted increased mitotic crossing-over frequencies in diploid strains heterozygous for several genetic markers including the ones involved in DNA repair and recombination mechanisms. Genetic markers of several heterozygous strains were individu`ally analyzed disclosing that some markers were especially sensitive to the treatments. These markers should be chosen as bio-indicators in the homozygotization index assay to better detect the recombinogenic/carcinogenic genomic effects of low-dose X-rays.

引用

页码：467 / 475

页数：9

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