A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology

被引:28
作者
Touloupakis, Eleftherios [1 ,2 ]
Boutopoulos, Christos [3 ]
Buonasera, Katia [2 ]
Zergioti, Ioanna [3 ]
Giardi, Maria Teresa [2 ]
机构
[1] Biosensor Srl, I-00060 Rome, Italy
[2] CNR, Inst Crystallog, I-00015 Rome, Italy
[3] Natl Tech Univ Athens, Dept Phys, Athens 15780, Greece
关键词
Laser printing; Biosensor; Photosynthesis; Herbicides; PHOTOSYSTEM-II; SENSOR RESPONSE; HERBICIDES; POLY(VINYLALCOHOL); IMMOBILIZATION; MICROARRAYS; COMPLEXES;
D O I
10.1007/s00216-012-5771-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335 +/- 13 nA for a thylakoid mass of 28 +/- 4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations.
引用
收藏
页码:3237 / 3244
页数:8
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