Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of β-Glycerophosphate

被引:26
作者
Liu, Mingyue [1 ]
Sun, Yao [2 ]
Liu, Yang [3 ]
Yuan, Mengtong [1 ]
Zhang, Zhihui [4 ]
Hu, Weiping [1 ]
机构
[1] Harbin Med Coll, Dept Prosthodont, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China
[2] Harbin Med Coll, Inst Hard Tissue Dev & Regenerat, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China
[3] Mianyang Cent Hosp, Dept Stomatol, Mianyang 150086, Sichuan, Peoples R China
[4] Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
关键词
MEPE; DSP; beta-glycerophosphate; dental pulp stem cells; odontoblast; IN-VITRO; BONE; MINERALIZATION; MATRIX; EXPRESSION; PROTEIN; TISSUE; GENE; MEPE; SIALOPHOSPHOPROTEIN;
D O I
10.3390/molecules17021219
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of beta-glycerophosphate (beta-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time-and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the beta-GP concentration, and there was also an influence on MEPE expression when different concentrations of beta-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, all experimental groups successfully generated mineralized nodules by Alizarin Red S and the 5 mM beta-GP group formed more mineralized nodules quantitated using the CPC extraction method. In conclusion, there is a significant modulation of the beta-GP during the differentiation of the DPSCs. The degree of odontoblast differentiation is beta-glycerophosphate concentration dependent. A low concentration of beta-GP (5 mM) has been shown to be the optimal concentration for stimulating the maturation of the DPSCs. Moreover, MEPE accompanied with DSP clearly demonstrates the degree of the differentiation.
引用
收藏
页码:1219 / 1232
页数:14
相关论文
共 36 条
[1]   Human dentin production in vitro [J].
About, I ;
Bottero, MJ ;
de Denato, P ;
Camps, J ;
Franquin, JC ;
Mitsiadis, TA .
EXPERIMENTAL CELL RESEARCH, 2000, 258 (01) :33-41
[2]   MEPE, the gene encoding a tumor-secreted protein in oncogenic hypophosphatemic osteomalacia, is expressed in bone [J].
Argiro, L ;
Desbarats, M ;
Glorieux, FH ;
Ecarot, B .
GENOMICS, 2001, 74 (03) :342-351
[3]   Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP) [J].
Bakopoulou, A. ;
Leyhausen, G. ;
Yolk, J. ;
Tsiftsoglou, A. ;
Garefis, P. ;
Koidis, P. ;
Geurtsen, W. .
ARCHIVES OF ORAL BIOLOGY, 2011, 56 (07) :709-721
[4]   Comparison of stem-cell-mediated osteogenesis and dentinogenesis [J].
Batouli, S ;
Miura, M ;
Brahim, J ;
Tsutsui, TW ;
Fisher, LW ;
Gronthos, S ;
Robey, PG ;
Shi, S .
JOURNAL OF DENTAL RESEARCH, 2003, 82 (12) :976-981
[5]   Odontoblast differentiation of human dental pulp cells in explant cultures [J].
Couble, ML ;
Farges, JC ;
Bleicher, F ;
Perrat-Mabillon, B ;
Boudeulle, M ;
Magloire, H .
CALCIFIED TISSUE INTERNATIONAL, 2000, 66 (02) :129-138
[6]   Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation [J].
d'Aquino, R. ;
Graziano, A. ;
Sampaolesi, M. ;
Laino, G. ;
Pirozzi, G. ;
De Rosa, A. ;
Papaccio, G. .
CELL DEATH AND DIFFERENTIATION, 2007, 14 (06) :1162-1171
[7]   Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo [J].
DSouza, RN ;
Cavender, A ;
Sunavala, G ;
Alvarez, J ;
Ohshima, T ;
Kulkarni, AB ;
MacDougall, M .
JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 (12) :2040-2049
[8]   Targeted disruption of the osteoblast/osteocyte factor 45 gene (OF45) results in increased bone formation and bone mass [J].
Gowen, LC ;
Petersen, DN ;
Mansolf, AL ;
Qi, H ;
Stock, JL ;
Tkalcevic, GT ;
Simmons, HA ;
Crawford, DT ;
Chidsey-Frink, KL ;
Ke, HZ ;
McNeish, JD ;
Brown, TA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (03) :1998-2007
[9]   Stem cell properties of human dental pulp stem cells [J].
Gronthos, S ;
Brahim, J ;
Li, W ;
Fisher, LW ;
Cherman, N ;
Boyde, A ;
DenBesten, P ;
Robey, PG ;
Shi, S .
JOURNAL OF DENTAL RESEARCH, 2002, 81 (08) :531-535
[10]   Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].
Gronthos, S ;
Mankani, M ;
Brahim, J ;
Robey, PG ;
Shi, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630