Surface and ultrastructural characterization of raw and pretreated switchgrass

被引:53
作者
Donohoe, Bryon S. [2 ]
Vinzant, Todd B. [2 ]
Elander, Richard T. [1 ]
Pallapolu, Venkata Ramesh [3 ]
Lee, Y. Y. [3 ]
Garlock, Rebecca J. [4 ,5 ]
Balan, Venkatesh [4 ,5 ]
Dale, Bruce E. [4 ,5 ]
Kim, Youngmi [6 ]
Mosier, Nathan S. [6 ]
Ladisch, Michael R. [6 ]
Falls, Matthew [7 ]
Holtzapple, Mark T. [7 ]
Sierra-Ramirez, Rocio [8 ]
Shi, Jian [9 ]
Ebrik, Mirvat A. [9 ]
Redmond, Tim [9 ]
Yang, Bin [9 ]
Wyman, Charles E. [9 ]
Hames, Bonnie [10 ]
Thomas, Steve [10 ]
Warner, Ryan E. [11 ]
机构
[1] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA
[2] Natl Renewable Energy Lab, Chem & Biosci Ctr, Golden, CO 80401 USA
[3] Auburn Univ, Dept Chem Engn, Auburn, AL 36849 USA
[4] Michigan State Univ, Dept Chem Engn & Mat Sci, Biomass Convers Res Lab, Lansing, MI 48910 USA
[5] Michigan State Univ, Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA
[6] Purdue Univ, Dept Agr & Biol Engn, LORRE, W Lafayette, IN 47907 USA
[7] Texas A&M Univ, Dept Chem Engn, College Stn, TX 77843 USA
[8] Univ Los Andes, Dept Chem Engn, Grp Convers Energia, Bogota, Colombia
[9] Univ Calif Riverside, Bourns Coll Engn, Dept Chem & Environm Engn, Ctr Environm Res & Technol, Riverside, CA 92507 USA
[10] Ceres Inc, Thousand Oaks, CA 91320 USA
[11] Genencor Inc, Palo Alto, CA 94304 USA
关键词
Pretreatment; Enzymatic hydrolysis; Biomass; Switchgrass; Microscopy; COMPARATIVE SUGAR RECOVERY; HOT-WATER PRETREATMENT; CORN STOVER; ENZYMATIC-HYDROLYSIS; LIME PRETREATMENT; POPLAR; DIGESTIBILITY; OPTIMIZATION;
D O I
10.1016/j.biortech.2011.03.092
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The US Department of Energy-funded Biomass Refining CAFI (Consortium for Applied Fundamentals and Innovation) project has developed leading pretreatment technologies for application to switchgrass and has evaluated their effectiveness in recovering sugars from the coupled operations of pretreatment and enzymatic hydrolysis. Key chemical and physical characteristics have been determined for pretreated switchgrass samples. Several analytical microscopy approaches utilizing instruments in the Biomass Surface Characterization Laboratory (BSCL) at the National Renewable Energy Laboratory (NREL) have been applied to untreated and CAFI-pretreated switchgrass samples. The results of this work have shown that each of the CAFI pretreatment approaches on switchgrass result in different structural impacts at the plant tissue, cellular, and cell wall levels. Some of these structural changes can be related to changes in chemical composition upon pretreatment. There are also apparently different structural mechanisms that are responsible for achieving the highest enzymatic hydrolysis sugar yields. (C) 2011 Published by Elsevier Ltd.
引用
收藏
页码:11097 / 11104
页数:8
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