Hybrid photodetector for single-molecule spectroscopy and microscopy

被引：23

作者：

Michalet, X. ^{[1
]}

Cheng, Adrian ^{[2
]}

Antelman, Joshua ^{[1
]}

Suyama, Motohiro ^{[3
]}

Arisaka, Katsushi ^{[2
]}

Weiss, Shimon ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, 607 Charles E Young Dr E, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Phys & Astron, 607 Charles E Young Dr E, Los Angeles, CA 90095 USA

[3] Hamamatsu Photon K K, Electron Tube Div, Iwata 4380193, Japan

来源：

SINGLE MOLECULE SPECTROSCOPY AND IMAGING | 2008年 / 6862卷

关键词：

hybrid photodetector; single-molecule; fluorescence; spectroscopy; microscopy; lifetime; afterpulsing; TCSPC; photocathode; FCS;

D O I：

10.1117/12.763449

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report benchmark tests of a new single-photon counting detector based on a GaAsP photocathode and an electron-bombarded avalanche photodiode developed by Hamamatsu Photonics. We compare its performance with those of standard Geiger-mode avalanche photodiodes. We show its advantages for FCS due to the absence of after-pulsing and for fluorescence lifetime measurements due to its excellent time resolution. Its large sensitive area also greatly simplifies setup alignment. Its spectral sensitivity being similar to that of recently introduced CMOS SPADs, this new detector could become a valuable tool for single-molecule fluorescence measurements, as well as for many other applications.

引用

页数：12

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