Detection of norovirus, sapovirus, and human astrovirus in fecal specimens using a multiplex reverse transcription-PCR with fluorescent dye-labeled primers

被引：9

作者：

Shigemoto, Naoki ^{[1
]}

Fukuda, Shinji ^{[1
]}

Tanizawa, Yukie ^{[1
]}

Kuwayama, Masaru ^{[1
]}

Ohara, Sachiko ^{[2
]}

Seno, Masato ^{[1
]}

机构：

[1] Hiroshima Prefectural Technol Res Inst, Ctr Publ Hlth & Environm, Minami Ku, Hiroshima 7340007, Japan

[2] Hiroshima Prefectural Meat Inspect Off, Hiroshima 7280025, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2011年 / 55卷 / 05期

关键词：

fluorescent dye-labeled primer; gastroenteric virus; multiplex RT-PCR; GENOGROUP-II; MOLECULAR EPIDEMIOLOGY; RT-PCR; VIRUS; SAMPLES; GASTROENTERITIS; OUTBREAKS; CHILDREN; INFANTS; JAPAN;

D O I：

10.1111/j.1348-0421.2011.00325.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We applied a multiplex reverse transcription-PCR with fluorescent dye-labeled primers (fluorescent multiplex RT-PCR) for noroviruses (NoV), sapovirus (SaV), and human astrovirus (HAstV) to diagnose 71 outbreaks of acute gastroenteritis during July 2007 and May 2010 in Hiroshima prefecture. In this assay, the green, red, yellow, and blue fluorescence for NoV genogroup I, NoV genogroup II, SaV, and HAstV, respectively, were indicated on an agarose gel under ultraviolet light. In 61 virus-positive outbreaks confirmed by fluorescent multiplex RT-PCR, detection rates of outbreaks for NoVs, SaV, and HAstV were 96.7%, 3.3%, and 0%, respectively.

引用

页码：369 / 372

页数：4

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