Comparison between aortic and sinusoidal liver endothelial cells as targets of hyperacute xenogeneic rejection in the pig to human combination

被引:20
作者
Cattan, P
Zhang, BM
Braet, F
Atia, N
Conti, F
Conjeaud, H
Weill, B
Chereau, C
Houssin, D
Calmus, Y
机构
[1] HOP COCHIN,SERV PROF YVES CHAPUIS,INSERM U283,F-75014 PARIS,FRANCE
[2] UNIV PARIS 05,FAC MED COCHIN PORT ROYAL,IMMUNOL LAB,PARIS,FRANCE
[3] UNIV PARIS 05,FAC MED COCHIN PORT ROYAL,LAB RECH CHIRURG,PARIS,FRANCE
[4] FREE UNIV BRUSSELS,CELL BIOL & HISTOL LAB,BRUSSELS,BELGIUM
关键词
D O I
10.1097/00007890-199609270-00018
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Endothelial cells of aortic origin are usually used in vitro as targets of hyperacute xenogeneic rejection, although endothelial cells from organs may have different properties. The sensitivities of aortic and liver endothelial cells to hyperacute xenogeneic rejection were compared in the pig to human combination. Sinusoidal liver endothelial cells were isolated and purified by collagenase perfusion of pig livers, sedimentation on a percoll gradient and selective adherence. Purity and viability of isolated liver endothelial cells after adherence were 85+/-6% and >95%, respectively, Endothelial cells from pig aortae (purity and viability >95%) were isolated by scraping. Immunoblotting analysis of xenoantigens on liver and aortic endothelial cell membranes preparations showed identical patterns, The strongest bands revealed by human IgM were located between 110 and 135 kD, while human IgG detected two major bands at 115 and 75 kD. The membrane expression of xenoantigens recognized by human sera, analyzed by flow cytometry, was significantly lower on liver than on aortic endothelial cells (IgM P=0.006; IgG: P=0.009). However, the complement-dependent cytotoxic activity of human sera was the same whether liver (54.5+/-1.4%) or aortic endothelial cells (50.0+/-4.2%) were used as targets. Taken together, those results allow the use of aortic instead of sinusoidal liver endothelial cells in the characterization of-pig antigens recognized by human natural antibodies.
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收藏
页码:803 / 810
页数:8
相关论文
共 43 条
  • [31] STEFFAN AM, 1981, CR ACAD SCI III-VIE, V292, P809
  • [32] PHAGOCYTOSIS, AN UNRECOGNIZED PROPERTY OF MURINE ENDOTHELIAL LIVER-CELLS
    STEFFAN, AM
    GENDRAULT, JL
    MCCUSKEY, RS
    MCCUSKEY, PA
    KIRN, A
    [J]. HEPATOLOGY, 1986, 6 (05) : 830 - 836
  • [33] INTERCELLULAR IMMUNE ADHESION MOLECULES IN HUMAN LIVER-TRANSPLANTS - OVERVIEW ON EXPRESSION PATTERNS OF LEUKOCYTE RECEPTOR AND LIGAND MOLECULES
    STEINHOFF, G
    BEHREND, M
    SCHRADER, B
    PICHLMAYR, R
    [J]. HEPATOLOGY, 1993, 18 (02) : 440 - 453
  • [34] DETECTION OF FACTOR-VIII-COAGULANT ANTIGEN IN HUMAN-LIVER TISSUE
    STEL, HV
    VANDERKWAST, TH
    VEERMAN, ECI
    [J]. NATURE, 1983, 303 (5917) : 530 - 532
  • [35] REMOVAL OF NATURAL HUMAN XENOANTIBODIES TO PIG VASCULAR ENDOTHELIUM BY PERFUSION OF BLOOD THROUGH PIG KIDNEYS AND LIVERS
    TUSO, PJ
    CRAMER, DV
    YASUNAGA, C
    COSENZA, CA
    WU, GD
    MAKOWKA, L
    [J]. TRANSPLANTATION, 1993, 55 (06) : 1375 - 1378
  • [36] TUSO PJ, 1992, TRANSPLANT P, V24, P596
  • [37] VANBOSSUYT H, 1988, SINUSOIDS HUMAN LIVE, P1
  • [38] VAUGHAN HA, 1993, TRANSPLANT P, V25, P2919
  • [39] REGULATION OF FATTY-ACID METABOLISM BY PANCREATIC HORMONES IN CULTURED HUMAN HEPATOCYTES
    VONS, C
    PEGORIER, JP
    GIRARD, J
    KOHL, C
    IVANOV, MA
    FRANCO, D
    [J]. HEPATOLOGY, 1991, 13 (06) : 1126 - 1130
  • [40] THE LIVER SIEVE - CONSIDERATIONS CONCERNING THE STRUCTURE AND FUNCTION OF ENDOTHELIAL FENESTRAE, THE SINUSOIDAL WALL AND THE SPACE OF DISSE
    WISSE, E
    DEZANGER, RB
    CHARELS, K
    VANDERSMISSEN, P
    MCCUSKEY, RS
    [J]. HEPATOLOGY, 1985, 5 (04) : 683 - 692