Hyperoxia by short-term promotes oxidative damage and mitochondrial dysfunction in rat brain

被引：14

作者：

Machado, Richard Simon ^{[1
]}

Tenfen, Leonardo ^{[1
]}

Joaquim, Larissa ^{[1
]}

Lanzzarin, Everton Venicius Rosa ^{[1
]}

Bernardes, Gabriela Costa ^{[1
]}

Bonfante, Sandra Regina ^{[1
]}

Mathias, Khiany ^{[1
]}

Biehl, Erica ^{[1
]}

Stork, Solange de Souza ^{[1
]}

Denicol, Tais ^{[1
]}

de Oliveira, Mariana Pacheco ^{[1
]}

da Silva, Mariella Reinol ^{[1
]}

Danielsk, Lucineia Gainski ^{[2
]}

de Quadros, Rafaella Willig ^{[3
]}

Rezin, Gislaine Tezza ^{[1
]}

Terra, Silvia Resende ^{[4
]}

Balsini, Jairo Nunes ^{[1
]}

Gava, Fernanda Frederico ^{[2
]}

Petronilho, Fabricia ^{[2
]}

机构：

[1] Univ South Santa Catarina, Grad Program Hlth Sci, Hlth Sci Unit, Lab Neurobiol Inflammatory & Metab Proc, Tubarao, SC, Brazil

[2] Univ Southern Santa Catarina, Grad Program Hlth Sci, Lab Expt Neurol, Criciuma, SC, Brazil

[3] Univ South Santa Catarina, Dept Med, Tubarao, SC, Brazil

[4] Fed Univ Rio Grande, Dept Biochem, Porto Alegre, Rio Grande do S, Brazil

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2022年 / 306卷

关键词：

Hyperoxia; Oxygen; Brain; Oxidative stress; NITRIC-OXIDE; NEONATAL HYPEROXIA; OXYGEN-THERAPY; CELL-DEATH; STRESS; IMPAIRMENT; NEUROINFLAMMATION; MINOCYCLINE; EXPOSURE; HYPOXIA;

D O I：

10.1016/j.resp.2022.103963

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Oxygen (O2) therapy is used as a therapeutic protocol to prevent or treat hypoxia. However, a high inspired fraction of O2 (FIO2) promotes hyperoxia, a harmful condition for the central nervous system (CNS). The present study evaluated parameters of oxidative stress and mitochondrial dysfunction in the brain of rats exposed to different FIO2. Male Wistar rats were exposed to hyperoxia (FIO2 40 % and 60 %) compared to the control group (FIO2 21 %) for 2 h. Oxidative stress, neutrophilic infiltration, and mitochondrial respiratory chain enzymes were determined in the hippocampus, striatum, cerebellum, cortex, and prefrontal cortex after O2 exposure. The animals exposed to hyperoxia showed increased lipid peroxidation, formation of carbonyl proteins, N/N concentration, and neutrophilic infiltration in some brain regions, like hippocampus, striatum, and cerebellum being the most affected. Furthermore, CAT activity and activity of mitochondrial enzyme complexes were also altered after exposure to hyperoxia. Rats exposed to hyperoxia showed increase in oxidative stress parameters and mitochondrial dysfunction in brain structures.

引用

页数：14

共 98 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2] Hyperoxia and Lungs: What We Have Learned From Animal Models [J].