Effect of the kanamycin resistance marker on stability of 2μ-based expression plasmids

被引:0
|
作者
Stankovic, Nada [1 ]
Vasiljevic, Branka [1 ]
Ljubliankic, G. [1 ]
机构
[1] Inst Mol Genet & Genet Engn, Belgrade 11010, Serbia
关键词
2 mu m plasmid; kanamycin resistance; penicillin G amidase; plasmid stability; Saccharomyces cerevisiae; selective markers; YEAST SACCHAROMYCES-CEREVISIAE; HIGH-EFFICIENCY TRANSFORMATION; ESCHERICHIA-COLI; GENE-EXPRESSION; SHUTTLE-VECTORS; 2-MU-M PLASMID; COPY-NUMBER; HIGH-LEVEL; PROVIDENCIA-RETTGERI; DNA;
D O I
10.2298/ABS0701001S
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this paper we describe the effect of the kanamycin resistance gene (Km(r)) on 2 mu m-based plasmid maintenance in Saccharomyces cerevisiae. The influence of this marker gene on the loss of the stable model-vectors proved to be constant, as well as independent of carbon source and culture growth rates. In strains for GAL(UAS) - driven heterologous protein production introduction of Km(r) resulted in curing of the yeast episomal plasmid (YEp) from the population in a small number of generations. Application of selective pressure on the strain producing recombinant penicillin G amidase (rPGA) did not provide the expected increase of protein yield. The influence of genetic elements for heterologous protein production on vector stability was examined, and the most destabilizing factors prove to be the presence and expression of the foreign gene..
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页码:1 / 12
页数:12
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