The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana

被引：5
作者：
Guo, Zhengfei [1 ,2 ]
Wang, Xiaoyu [2 ]
Hu, Zhubing [2 ]
Wu, Chengyun [2 ]
Shen, Zhenguo [1 ]
机构：
[1] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China
[2] Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2021年 / 578卷
基金：
中国国家自然科学基金;
关键词：
PPR protein; ccmFn-1; editing; Cytochrome C; Root development; High temperature response; SEED DEVELOPMENT; SENSITIVE MUTANTS; COMPLEX; INTERACTS; PROVIDES; GROWTH;
D O I：
10.1016/j.bbrc.2021.09.022
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pentatricopeptide repeat (PPR) proteins are a large family in land plants that play a role in organellular RNA processing, editing, and splicing. Here, we identify an Arabidopsis thaliana mutant, gend1-1, which exhibits a short root phenotype with reduced meristem size and cell numbers. Positional cloning of GEND1 revealed that it encodes a PPR protein, and functional analysis showed that GEND1 can bind and edit mitochondrial ccmFn-1 mRNA, causing gend1 mutants to have decreased levels of cytochrome C. GEND1 was up-regulated by high temperature conditions, to which gend1 mutants were hypersensitive. Analysis of a set of PPR mutants under high temperature showed that mutants with defects in cytochrome C had comparable temperature sensitivity to gend1. Collectively, these results suggest that cytochrome C plays an important role in root development and high temperature response in Arabidopsis. (C) 2021 Elsevier Inc. All rights reserved.
引用
收藏
页码：63 / 69
页数：7
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