A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies

被引：22
作者：
An, S. [2 ,3 ,4 ]
Ma, D. [2 ,4 ]
Wei, J. F. [1 ]
Yang, X. [2 ,3 ]
Yang, H. W. [1 ]
Yang, H. [2 ,4 ]
Xu, X. [2 ]
He, S. [1 ]
Lai, R. [2 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 1, Clin Res Ctr, Nanjing 210029, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Biotoxin Units, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming 650223, Yunnan, Peoples R China
[3] Univ Sci & Technol China, Sch Life Sci, Hefe, Peoples R China
[4] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
来源：
ALLERGY | 2011年 / 66卷 / 11期
关键词：
apyrase; basophil; CD63; horsefly allergen; insect allergy; 5'-NUCLEOTIDASE FAMILY; ANOPHELES-GAMBIAE; AEDES-AEGYPTI; PURIFICATION; APYRASE; VENOM; EXPRESSION; PROTEIN; IDENTIFICATION; HYALURONIDASE;
D O I：
10.1111/j.1398-9995.2011.02683.x
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Background: Horsefly sting causes allergic reactions in human body. However, our knowledge on horsefly allergens remains poor. Objectives: To identify the novel horsefly allergens and characterize their properties. Methods: A native allergen protein Tab y 1 (apyrase) was purified from the salivary glands of the horsefly Tabanus yao Macquart by gel filtration and ion exchange chromatography. Its sequence was determined by Edman degradation and cDNA cloning. Its allergenicity was assessed by immunoblotting for specific IgE, basophil activation test, skin prick test (SPT), and competitive enzyme-linked immunosorbent assay (ELISA). Results: Tab y 1 showed a single diffusion band of 70 kDa on SDS-PAGE. Seventy percent (7/10) of patients with horsefly allergy tested positive to Tab y 1 in SPT; sera from 81% (30/37) of patients reacted to Tab y 1 on western blots. Purified Tab y 1 reduced approximately 42% sera IgE reactivity to horsefly salivary gland extract on a competitive ELISA. Tab y 1 upregulated the expression of CD63 and CCR3 on passively sensitized basophils by up to approximately 4.9-fold. Tab y 1 also showed enzymatic activity to hydrolyze ATP and ADP, and potent antiplatelet aggregation and antithrombotic activities. Conclusion: The current work identified a novel major allergen of horsefly, Tab y 1, with antiplatelet aggregation and antithrombotic activities, which implicates Tab y 1 in helping horseflies suck host blood, meanwhile causing allergy in their human hosts.
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页码：1420 / 1427
页数：8
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