CYP1A1 is a target of miR-892a-mediated post-transcriptional repression

被引：55

作者：

Choi, Yeong Min ^{[1
,2
]}

An, Sungkwan ^{[1
,2
,3
]}

Lee, Eun-Mee ^{[1
,2
]}

Kim, Karam ^{[1
,2
]}

Choi, Sung Jin ^{[1
,2
]}

Kim, Ju Sub ^{[4
]}

Jang, Hyun Hee ^{[5
]}

An, In-Sook ^{[1
,2
,3
]}

Bae, Seunghee ^{[1
,2
]}

机构：

[1] Konkuk Univ, Funct Genoproteome Res Ctr, Seoul 143701, South Korea

[2] LIFEnGENE Inc, Seoul 143701, South Korea

[3] Konkuk Univ, Aesthet & Cosmetol Res Inst, Seoul 143701, South Korea

[4] Sangji Youngseo Coll, Dept Cosmetol, Wonju 220713, Gwangwon Do, South Korea

[5] Kyungbok Univ, Sch Cosmetol, Pocheon Si 487717, Gyeonggi Do, South Korea

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2012年 / 41卷 / 01期

关键词：

cytochrome P450 1A1; benzo(a)pyrene; microRNA; miR-892a; cell viability; CELL-PROLIFERATION; MICRORNA TARGETS; EXPRESSION; BENZO(A)PYRENE; ACTIVATION; DROSOPHILA; CANCER; GENES; MICE;

D O I：

10.3892/ijo.2012.1418

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cytochrome P450 1A1 (CYP1A1) is a member of the cytochrome p450 enzyme family, which is involved in the metabolisms of carcinogenic metabolites, such as benzo(a) pyrene. In this study, we identified miR-892a as a negative regulator of CYP1A1 expression. Luciferase assays revealed a sequence in the 3'-untranslated region of CYP1A1 that displayed a perfect match with miR-892a, and revealed that this sequence was a specific miR-892a target site. The overexpression of miR-892a inhibited the expression of the CYP1A1 protein, and the miR-892a antagonist increased CYP1A1 expression. Of note, benzo(a)pyrene, a major inducer of CYP1A1 transcription, decreased the expression of miR-892a. Moreover, the miR-892a-induced CYP1A1 repression inhibited the benzo(a) pyrene-mediated decrease in cell viability. These data provide insight into the CYP1A1 regulatory network.

引用

页码：331 / 336

页数：6

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