Colwellia sediminilitoris sp nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, motile and rod-shaped or ovoid bacterial strain, designated YSM-23(T), was isolated from a tidal flat on the South Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain YSM-23(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 1.0-2.0% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain YSM-23(T) represented a member of the genus Colwellia. Strain YSM-23(T) exhibited 16S rRNA gene sequence similarity values of 98.0, 97.4 and 97.3% to the type strains of Colwellia aestuarii, Colwellia polaris and Colwellia chukchiensis, respectively, and of 94.5-96.8% to the type strains of the other species of the genus Colwellia. Strain YSM-23(T) contained Q-8 as the predominant ubiquinone and summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c) as the major fatty acid. The major polar lipids detected in strain YSM-23T were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain YSM-23(T) was 43.8 +/- 0.08 mol% and its DNA-DNA relatedness values with the type strain of C. aestuarii, C. polaris and C. chukchiensis were 10 +/- 3.5-22 +/- 4.9 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain YSM-23(T) is separated from species of the genus Colwellia with validly published names. On the basis of the data presented, strain YSM-23(T) is considered to represent a novel species of the genus Colwellia, for which the name Colwellia sediminilitoris sp. nov. is proposed. The type strain is YSM-23(T) (= KCTC 52213(T) = NBRC 111994(T)).