Simultaneous Purification and Site-Specific Modification of Pyrroline-Carboxy-Lysine Proteins

被引:1
|
作者
Chiu, Hsien-Po [1 ,2 ]
Gruenewald, Jan [1 ,2 ]
Hao, Xueshi [1 ,2 ]
Brock, Ansgar [1 ,2 ]
Okach, Linda [1 ,2 ]
Uno, Tetsuo [1 ,2 ]
Geierstanger, Bernhard H. [1 ,2 ]
机构
[1] Genom Inst, San Diego, CA 92121 USA
[2] Novartis Res Fdn, San Diego, CA 92121 USA
关键词
affinity tags; protein engineering; protein expression; protein purification; pyrroline-carboxy-lysine; AFFINITY-CHROMATOGRAPHY; STEP PURIFICATION; ESCHERICHIA-COLI; FUSION; PYRROLYSINE; BIOSYNTHESIS; SELECTIVITY; RESOLUTION; PEPTIDE; NMR;
D O I
10.1002/cbic.201100684
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:364 / 366
页数:3
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