Simultaneous Purification and Site-Specific Modification of Pyrroline-Carboxy-Lysine Proteins

被引:1
|
作者
Chiu, Hsien-Po [1 ,2 ]
Gruenewald, Jan [1 ,2 ]
Hao, Xueshi [1 ,2 ]
Brock, Ansgar [1 ,2 ]
Okach, Linda [1 ,2 ]
Uno, Tetsuo [1 ,2 ]
Geierstanger, Bernhard H. [1 ,2 ]
机构
[1] Genom Inst, San Diego, CA 92121 USA
[2] Novartis Res Fdn, San Diego, CA 92121 USA
关键词
affinity tags; protein engineering; protein expression; protein purification; pyrroline-carboxy-lysine; AFFINITY-CHROMATOGRAPHY; STEP PURIFICATION; ESCHERICHIA-COLI; FUSION; PYRROLYSINE; BIOSYNTHESIS; SELECTIVITY; RESOLUTION; PEPTIDE; NMR;
D O I
10.1002/cbic.201100684
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:364 / 366
页数:3
相关论文
共 50 条
  • [21] Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry
    Reddi, Rambabu N.
    Rogel, Adi
    Resnick, Efrat
    Gabizon, Ronen
    Prasad, Pragati Kishore
    Gurwicz, Neta
    Barr, Haim
    Shulman, Ziv
    London, Nir
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (48) : 20095 - 20108
  • [22] Challenges of site-specific selenocysteine incorporation into proteins by Escherichia coli
    Fu, Xian
    Soll, Dieter
    Sevostyanova, Anastasia
    RNA BIOLOGY, 2018, 15 (4-5) : 461 - 470
  • [23] Site-Specific Dual Labeling of Proteins on Cysteine Residues with Chlorotetrazines
    Canovas, Coline
    Moreau, Mathieu
    Bernhard, Claire
    Oudot, Alexandra
    Guillemin, Melanie
    Denat, Franck
    Goncalves, Victor
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (33) : 10646 - 10650
  • [24] Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using SortaseA and Elastin-like Polypeptides
    Bellucci, Joseph J.
    Amiram, Miriam
    Bhattacharyya, Jayanta
    McCafferty, Dewey
    Chilkoti, Ashutosh
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (13) : 3703 - 3708
  • [25] Site-specific protein modification by genetic encoded disulfide compatible thiols
    Ling, Xinyu
    Chen, Heqi
    Zheng, Wei
    Chang, Liying
    Wang, Yong
    Liu, Tao
    CHINESE CHEMICAL LETTERS, 2020, 31 (01) : 163 - 166
  • [26] Site-Specific Modification of Native IgGs with Flexible Drug-Load
    Wehrmueller, Joeri E.
    Frei, Julia C.
    Hechler, Torsten
    Kulke, Michael
    Pahl, Andreas
    Behe, Martin
    Schibli, Roger
    Spycher, Philipp R.
    CHEMBIOCHEM, 2024,
  • [27] Site-Specific Modification of the Anticancer and Antituberculosis Polyether Salinomycin by Biosynthetic Engineering
    Luhavaya, Hanna
    Williams, Simon R.
    Hong, Hui
    de Oliveira, Luciana Gonzaga
    Leadlay, Peter F.
    CHEMBIOCHEM, 2014, 15 (14) : 2081 - 2085
  • [28] Simultaneous Site-Specific Dual Protein Labeling Using Protein Prenyltransferases
    Zhang, Yi
    Blanden, Melanie J.
    Sudheer, Ch.
    Gangopadhyay, Soumyashree A.
    Rashidian, Mohammad
    Hougland, James L.
    Distefano, Mark D.
    BIOCONJUGATE CHEMISTRY, 2015, 26 (12) : 2542 - 2553
  • [29] Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine
    Syed, Junetha
    Palani, Saravanan
    Clarke, Scott T.
    Asad, Zainab
    Bottrill, Andrew R.
    Jones, Alexandra M. E.
    Sampath, Karuna
    Balasubramanian, Mohan K.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (10)
  • [30] Site-Specific Incorporation of Fluorinated Prolines into Proteins and Their Impact on Neighbouring Residues
    Elena-Real, Carlos A.
    Urbanek, Annika
    Sagar, Amin
    Mohanty, Priyesh
    Levy, Geraldine
    Morato, Anna
    Fournet, Aurelie
    Allemand, Frederic
    Sibille, Nathalie
    Mittal, Jeetain
    Sinnaeve, Davy
    Bernado, Pau
    CHEMISTRY-A EUROPEAN JOURNAL, 2025, 31 (06)