Differential utilisation of ceramide during replication of the flaviviruses West Nile and dengue virus

被引：53
作者：
Aktepe, Turgut E. [1 ]
Pham, Helen [2 ]
Mackenzie, Jason M. [1 ]
机构：
[1] Univ Melbourne, Dept Microbiol & Immunol, Peter Doherty Inst Infect & Immun, Melbourne, Vic, Australia
[2] La Trobe Univ, Dept Microbiol, Melbourne, Vic, Australia
来源：
VIROLOGY | 2015年 / 484卷
基金：
英国医学研究理事会;
关键词：
West Nile virus; Dengue virus; Virus replication; Ceramide; 3-DIMENSIONAL ARCHITECTURE; INFECTED-CELLS; RNA; SPHINGOSINE; SITES; NS1; INVOLVEMENT; INHIBITION; METABOLISM; MEMBRANES;
D O I：
10.1016/j.virol.2015.06.015
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
It is well established that + ssRNA viruses manipulate cellular lipid homoeostasis and distribution to facilitate efficient replication. Here, we show that the cellular lipid ceramide is redistributed to the West Nile virus strain Kunjin virus (WNVKUN) replication complex (RC) but not to the dengue virus serotype 2 strain New Guinea C (DENVNGC) RC. We show that prolonged chemical inhibition of serine palmitoyltransferase with myriocin had a significant deleterious effect on WNVKUN replication but enhanced DENVNGC replication. Additionally, inhibition of ceramide synthase with Fumonisin B-1 had a detrimental effect on WNVKUN replication and release of infectious virus particles but contrastingly an enhancing effect on DENVNGC replication and virus production. These observations suggest that ceramide production via the de nova and salvage pathway is a requirement for WNVKUN replication but inhibitory for DENVNGC replication. Thus, although these two viruses are from the same genus, they have a differential ceramide requirement for replication. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 250
页数：10
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