Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells

被引：18
作者：
Lee, Ha-reum [2 ]
Yoon, Sun Young [2 ]
Song, Seok Bean [2 ]
Park, Yoorim [2 ]
Kim, Tae Sung [3 ]
Kim, Seonghan [4 ]
Hur, Dae Young [4 ]
Song, Hyun Keun [4 ]
Park, Hyunjeong [1 ]
Cho, Daeho [2 ]
机构：
[1] Catholic Univ Korea, Dept Dermatol, St Marys Hosp, Coll Med, Seoul, South Korea
[2] Sookmyung Womens Univ, Dept Life Sci, Seoul, South Korea
[3] Korea Univ, Sch Life Sci & Biotechnol, Seoul, South Korea
[4] Inje Univ, Coll Med, Dept Anat, Pusan, South Korea
来源：
IMMUNOBIOLOGY | 2011年 / 216卷 / 10期
基金：
新加坡国家研究基金会;
关键词：
Interleukin-18; Natural killer cells; Thymosin beta 4; Interferon-gamma; NATURAL-KILLER-CELLS; IFN-GAMMA; ACTIN CYTOSKELETON; PROINFLAMMATORY CYTOKINE; INFLAMMATORY MEDIATORS; IMMUNE-RESPONSES; GENE-TRANSFER; T-CELLS; ACTIVATION; INNATE;
D O I：
10.1016/j.imbio.2011.04.002
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Thymosin beta 4 (T beta 4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates T beta 4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in T beta 4 expression and if enhanced T beta 4 influences IL-18-mediated interferon-gamma (IFN-gamma) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of T beta 4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous T beta 4 stimulated IFN-gamma expression and secretion. Additionally, pretreatment with an inhibitor for T beta 4 decreased IL-18-enhanced IFN-gamma secretion, and transfection with T beta 4-specific short hairpin RNA resulted in reduction of IFN-gamma production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that T beta 4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-gamma secretion in NK cells. (c) 2011 Elsevier GmbH. All rights reserved.
引用
收藏
页码：1155 / 1162
页数：8
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