Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

被引:17
作者
Schasfoort, Richard B. M. [1 ,2 ]
Abali, Fikri [1 ]
Stojanovic, Ivan [1 ,2 ]
Vidarsson, Gestur [3 ]
Terstappen, Leon W. M. M. [1 ]
机构
[1] Univ Twente, Med Cell BioPhys Grp MCBP, NL-7500 AE Enschede, Netherlands
[2] Interfluid BV, NL-7483 AL Haaksbergen, Netherlands
[3] Sanquin Res & Landsteiner Lab, Dept Expt Immunohematol, NL-1066 CX Amsterdam, Netherlands
来源
BIOSENSORS-BASEL | 2018年 / 8卷 / 04期
关键词
surface plasmon resonance; cytometry; SPR imaging; single-cell secretion; SURFACE-PLASMON RESONANCE; ANTIBODY-PRODUCTION; HYBRIDOMA CELLS; LIVING CELLS; CHIP; ANTIGENS; GROWTH;
D O I
10.3390/bios8040102
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.
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页数:11
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