Single cell gel electrophoresis assay: methodology and applications

被引：410
|
作者：
Rojas, E [1 ]
Lopez, MC [1 ]
Valverde, M [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Genet & Toxicol Ambiental, Lab Genet & Toxicol Mol, Mexico City 04510, DF, Mexico
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 722卷 / 1-2期
关键词：
reviews; single cell gel electrophoresis; Comet assay; DNA;
D O I：
10.1016/S0378-4347(98)00313-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukariotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review the studies published to date about the principles, the basic methodology with currently used variations. We also explore the applications of this assay in: genotoxicology, clinical area, DNA repair studies, environmental biomonitoring and human monitoring. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：225 / 254
页数：30
相关论文
共 50 条
  • [1] Single cell gel electrophoresis assay: Methodology and applications
    Rojas, E.
    Lopez, M.C.
    Valverde, M.
    Journal of Chromatography B, 1999, 722 (1-2): : 225 - 254
  • [2] Automated measurements of tails in, the single cell gel electrophoresis assay
    Rivest, JF
    Tang, M
    McLean, J
    Johnson, F
    JOINT CONFERENCE - 1996: IEEE INSTRUMENTATION AND MEASUREMENT TECHNOLOGY CONFERENCE & IMEKO TECHNICAL COMMITTEE 7, CONFERENCE PROCEEDINGS, VOLS I AND II: QUALITY MEASUREMENTS: THE INDISPENSABLE BRIDGE BETWEEN THEORY AND REALITY (NO MEASUREMENTS? NO SCIENCE!), 1996, : 111 - 114
  • [3] Single cell gel electrophoresis assay in the evaluation of metal carcinogenicity
    Rojas, E
    Valverde, M
    Sordo, M
    AltamiranoLozano, M
    OstroskyWegman, P
    METAL IONS IN BIOLOGY AND MEDICINE, VOL 4, 1996, 4 : 375 - 377
  • [4] THE SINGLE-CELL GEL-ELECTROPHORESIS ASSAY (COMET ASSAY) - A EUROPEAN REVIEW
    MCKELVEYMARTIN, VJ
    GREEN, MHL
    SCHMEZER, P
    POOLZOBEL, BL
    DEMEO, MP
    COLLINS, A
    MUTATION RESEARCH, 1993, 288 (01): : 47 - 63
  • [5] Single cell gel electrophoresis assay: A DNA breakage detection technique
    Qin, CH
    Shen, JY
    Huang, S
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 1995, 22 (06) : 517 - 520
  • [6] Single-cell gel electrophoresis (the comet assay): Loops or fragments?
    Shaposhnikov, Sergey A.
    Salenko, Veniamin B.
    Brunborg, Gunnar
    Nygren, Jonas
    Collins, Andrew R.
    ELECTROPHORESIS, 2008, 29 (14) : 3005 - 3012
  • [7] A MODIFIED RAPID SINGLE-CELL GEL-ELECTROPHORESIS ASSAY
    TAJ, S
    VADHANAM, VM
    NAGARAJAN, B
    MEDICAL SCIENCE RESEARCH, 1995, 23 (09): : 641 - 643
  • [8] Convolutional Neural Network for Segmentation of Single Cell Gel Electrophoresis Assay
    Ruz-Suarez, Daniel
    Martin-Gonzalez, Anabel
    Brito-Loeza, Carlos
    Leonor Pacheco-Pantoja, Elda
    INTELLIGENT COMPUTING SYSTEMS (ISICS 2022), 2022, 1569 : 57 - 68
  • [9] The application of single cell gel electrophoresis or comet assay to human monitoring studies
    Valverde, M
    Ostrosky-Wegman, P
    Rojas, E
    Fortoul, T
    Meneses, F
    Ramírez, M
    Díaz-Barriga, F
    Cebrian, M
    SALUD PUBLICA DE MEXICO, 1999, 41 : S109 - S113
  • [10] Single cell gel electrophoresis assay:: a new technique for human biomonitoring studies
    Kassie, F
    Parzefall, W
    Knasmüller, S
    MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2000, 463 (01) : 13 - 31
12345