Single cell gel electrophoresis assay: methodology and applications

被引：410
作者：
Rojas, E [1 ]
Lopez, MC [1 ]
Valverde, M [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Dept Genet & Toxicol Ambiental, Lab Genet & Toxicol Mol, Mexico City 04510, DF, Mexico
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 722卷 / 1-2期
关键词：
reviews; single cell gel electrophoresis; Comet assay; DNA;
D O I：
10.1016/S0378-4347(98)00313-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukariotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review the studies published to date about the principles, the basic methodology with currently used variations. We also explore the applications of this assay in: genotoxicology, clinical area, DNA repair studies, environmental biomonitoring and human monitoring. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：225 / 254
页数：30
相关论文
共 292 条
  • [1] Photochemical and photobiological studies of a furonaphthopyranone as a benzo-spaced psoralen analog in cell-tree and cellular DNA
    Adam, W
    Mielke, K
    SahaMoller, CR
    Moller, M
    Stopper, H
    Hutterer, R
    Schneider, FW
    Ballmaier, D
    Epe, B
    Gasparro, FF
    Cheng, XS
    Kagan, J
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (01) : 46 - 54
  • [2] Afanas'ev G G, 1997, Tsitologiia, V39, P740
  • [3] AFANESEV GG, 1998, TSITOLOGIYA, V39, P74073
  • [4] Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays
    Alapetite, C
    Wachter, T
    Sage, E
    Moustachi, E
    [J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1996, 69 (03) : 359 - 369
  • [5] AltamiranoLozano M, 1996, TERATOGEN CARCIN MUT, V16, P7, DOI 10.1002/(SICI)1520-6866(1996)16:1<7::AID-TCM2>3.0.CO
  • [6] 2-M
  • [7] In vitro genotoxic effects of hard metal particles assessed by alkaline single cell gel and elution assays
    Anard, D
    KirschVolders, M
    Elhajouji, A
    Belpaeme, K
    Lison, D
    [J]. CARCINOGENESIS, 1997, 18 (01) : 177 - 184
  • [8] The dynamics of technical innovation: The evolution and development of information technology - Duysters,G
    Andersen, HB
    [J]. BUSINESS HISTORY, 1997, 39 (02) : 161 - 163
  • [9] Somatic and germ cell effects in rats and mice after treatment with 1,3-butadiene and its metabolites, 1,2-epoxybutene and 1,2,3,4-diepoxybutane
    Anderson, D
    Dobrzynka, MM
    Jackson, LI
    Yu, TW
    Brinkworth, MH
    [J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1997, 391 (03) : 233 - 242
  • [10] Anderson D, 1997, TERATOGEN CARCIN MUT, V17, P29, DOI 10.1002/(SICI)1520-6866(1997)17:1<29::AID-TCM5>3.0.CO
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