Correlation between MALDI-TOF Vitek-MS™ system and conventional identification methods of gastrointestinal infection causing bacteria

被引:0
作者
Ruiz de Alegria-Puig, Carlos [1 ]
Aguirre-Quinonero, Amaia [1 ]
Aguero-Balbin, Jesus [1 ,2 ]
Pia Roiz-Mesones, Ma [1 ]
Martinez-Martinez, Luis [1 ,2 ]
机构
[1] Hosp Univ Marques de Valdecilla IDIVAL, Microbiol Serv, Avda Valdecilla S-N, Santander 39008, Spain
[2] Univ Cantabria, Dept Biol Mol, Santander, Spain
关键词
Maldi-tof; Yersinia; Aeromonas; Helicobacter; Salmonella; FLIGHT MASS-SPECTROMETRY; MS; AEROMONAS; TIME;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Introduction. Rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry has shown to be effective and fast for the identification of microorganisms. The objective of this study was to evaluate the correlation between Vitek-MS (TM) and conventional methods for bacterial identification causing gastrointestinal infection. Material and methods. A total of 329 gastrointestinal pathogens were identified using Vitek-MS (TM) (v2 SARAMIS MS-ID, bioMerieux, Marcy-I'Etoile, France) and routine diagnostic methods simultaneously. In cases of discrepancy 16SrRNA gene sequencing was performed. Results. The correlation between Vitek-MSTM and diagnostic methods was 100% except for Yersinia enterocolitica (94.1%), Helicobacter pylori (10%) and Aeromonas veronii (0 %). Conclusions. Vitek-MS (TM) is a quick and useful method for identification of enterophatogenic bacteria. It is necessary to improve the performance of the system for the identification of H. pylori and A. veronii.
引用
收藏
页码:265 / 268
页数:4
相关论文
共 11 条
[1]   Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology [J].
Clark, Andrew E. ;
Kaleta, Erin J. ;
Arora, Amit ;
Wolk, Donna M. .
CLINICAL MICROBIOLOGY REVIEWS, 2013, 26 (03) :547-603
[2]   A comparison of gastroenteritis in a general practice-based study and a community-based study [J].
De Wit, MAS ;
Kortbeek, LM ;
Koopmans, MPG ;
De Jager, CJ ;
Wannet, WJB ;
Bartelds, AIM ;
Van Duynhoven, YTHP .
EPIDEMIOLOGY AND INFECTION, 2001, 127 (03) :389-397
[3]   Comparison of MALDI-TOF MS, gene sequencing and the Vitek 2 for identification of seventy-three clinical isolates of enteropathogens [J].
Deng, Jiankai ;
Fu, Liang ;
Wang, Ruilian ;
Yu, Nan ;
Ding, Xixia ;
Jiang, Lingxiao ;
Fang, Yanping ;
Jiang, Changhong ;
Lin, Lijuan ;
Wang, Ying ;
Che, Xiaoyan .
JOURNAL OF THORACIC DISEASE, 2014, 6 (05) :539-544
[4]   Evaluation of mass spectrometry for the identification of clinically interesting yeasts [J].
Galan, Fatima ;
Garcia-Agudo, Lidia ;
Guerrero, Inmaculada ;
Marin, Pilar ;
Garcia-Tapia, Ana ;
Garcia-Martos, Pedro ;
Rodriguez-Iglesias, Manuel .
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2015, 33 (06) :372-378
[5]   Optimization of Campylobacter growth conditions for further identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [J].
Martiny, Delphine ;
Visscher, Alain ;
Catry, Boudewijn ;
Chatellier, Sonia ;
Vandenberg, Olivier .
JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 94 (03) :221-223
[6]   rpoB sequence analysis as a novel basis for bacterial identification [J].
Mollet, C ;
Drancourt, M ;
Raoult, D .
MOLECULAR MICROBIOLOGY, 1997, 26 (05) :1005-1011
[7]   Multiplex PCR for identification of Campylobacter coli and Campylobacter jejuni from pure cultures and directly on stool samples [J].
Persson, S ;
Olsen, KEP .
JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (11) :1043-1047
[8]  
Podewils Laura Jean, 2004, Semin Pediatr Infect Dis, V15, P155, DOI 10.1053/j.spid.2004.05.008
[9]   AUTOSCAN-4 SYSTEM FOR IDENTIFICATION OF GRAM-NEGATIVE BACILLI [J].
RHODEN, DL ;
SMITH, PB ;
BAKER, CN ;
SCHABLE, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (06) :915-918
[10]   Comparison of MALDI-TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates [J].
Shin, Hee Bong ;
Yoon, Jihoon ;
Lee, Yangsoon ;
Kim, Myung Sook ;
Lee, Kyungwon .
YONSEI MEDICAL JOURNAL, 2015, 56 (02) :550-555