Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

被引：131

作者：

Ke, Guoliang ^{[1
,2
,3
]}

Liu, Minghui ^{[1
,2
]}

Jiang, Shuoxing ^{[1
,2
]}

Qi, Xiaodong ^{[1
,2
]}

Yang, Yuhe Renee ^{[1
,2
]}

Wootten, Shaun ^{[1
,2
]}

Zhang, Fei ^{[1
,2
]}

Zhu, Zhi ^{[3
]}

Liu, Yan ^{[1
,2
]}

Yang, Chaoyong James ^{[3
]}

Yan, Hao ^{[1
,2
]}

机构：

[1] Arizona State Univ, Biodesign Inst, Ctr Mol Design & Biomimet, Tempe, AZ 85287 USA

[2] Arizona State Univ, Sch Mol Sci, Tempe, AZ 85287 USA

[3] Xiamen Univ, MOE Key Lab Spectrochem Anal & Instrumentat, State Key Lab Phys Chem Solid Surfaces,Coll Chem, Collaborat Innovat Ctr Chem Energy Mat,Dept Biol, Xiamen 361005, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 26期

基金：

美国国家科学基金会;

关键词：

DNA origami; enzymes; NADH; self-assembly; substrate channeling; NANOSTRUCTURES; COMPLEXES; ART;

D O I：

10.1002/anie.201603183

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.

引用

收藏

页码：7483 / 7486

页数：4

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