Development and Application of Est-Ssr Markers in Quails

被引：19
|
作者：
Bai, J. Y. [1 ]
Pang, Y. Z. [1 ]
Qi, Y. X. [1 ]
Zhang, X. H. [1 ]
Yun, X. Y. [1 ]
机构：
[1] Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471003, Peoples R China
来源：
BRAZILIAN JOURNAL OF POULTRY SCIENCE | 2016年 / 18卷
关键词：
Chinese yellow quail; EST-SSR; Bioinformatics; Genetic diversity;
D O I：
10.1590/1806-9061-2015-0124
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Aiming at accelerating the application of molecular markers in the genetic improvement of quails, six EST-SSR markers were successfully developed using a bioinformatics method. Polymorphisms of three quail populations (Chinese yellow quail, China black quail and Korean quail) were detected. The results showed that there were 2-6 alleles in six EST-SSR markers. Mean polymorphism information contents of Chinese yellow quails, Chinese black quails and Korean quails were determined as 0.5451, 0.4962 and 0.4937, respectively. Average heterozygosity valuesof 0.6134, 0.5759 and 0.5613 were calculated. Among the six EST-SSR markers, three were highly polymorphic, and the other three were moderately polymorphic. The newly-developed six EST-SSR markers may be used to determine the genetic diversity of quails. The six EST-SSR markers identified were related to carbohydrate metabolism and melanin synthesis, but their specific mechanisms need to be further analyzed.
引用
收藏
页码：27 / 31
页数：5
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