Exogenous Calcium Improves Viability of Biocontrol Yeasts Under Heat Stress by Reducing ROS Accumulation and Oxidative Damage of Cellular Protein

被引：48
作者：
An, Bang [1 ,2 ]
Li, Boqiang [1 ]
Qin, Guozheng [1 ]
Tian, Shiping [1 ]
机构：
[1] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
来源：
CURRENT MICROBIOLOGY | 2012年 / 65卷 / 02期
基金：
中国国家自然科学基金;
关键词：
BIOLOGICAL-CONTROL; SACCHAROMYCES-CEREVISIAE; DEBARYOMYCES-HANSENII; POSTHARVEST DISEASES; PENICILLIUM-EXPANSUM; PREHARVEST; RESISTANCE; TOLERANCE; EFFICACY; ENZYMES;
D O I：
10.1007/s00284-012-0133-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium in culture medium was beneficial for enhancing the tolerance of the biocontrol yeasts to heat stress. The possible mechanism of calcium improving the viability of yeasts was attributed to enhancement of antioxidant enzyme activities, decrease in ROS accumulation and reduction of oxidative damage of intracellular protein in yeast cells under heat stress. D. hansenii is more sensitive to calcium as compared to P. membranaefaciens. Our results suggest that application of exogenous calcium combined with biocontrol yeasts is a practical approach for the control of postharvest disease in fruit.
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收藏
页码：122 / 127
页数：6
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