Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures

被引：78

作者：

Kai, Guoyin ^{[1
]}

Liao, Pan ^{[1
]}

Xu, Hui ^{[1
]}

Wang, Jing ^{[1
]}

Zhou, Congcong ^{[1
]}

Zhou, Wei ^{[1
]}

Qi, Yaping ^{[1
]}

Xiao, Jianbo ^{[1
]}

Wang, Yuliang ^{[2
]}

Zhang, Lin ^{[3
]}

机构：

[1] Shanghai Normal Univ, Coll Life & Environm Sci, Plant Biotechnol Lab, Shanghai 200234, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Agr & Biol, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Shanghai 200030, Peoples R China

[3] Shaoxing Peoples Hosp, Dept Pharm, Shaoxing 312000, Peoples R China

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2012年 / 34卷 / 04期

关键词：

Dan Shen; Bioactive compounds; Gene expression; Metabolic engineering; Elicitors; GERANYLGERANYL DIPHOSPHATE SYNTHASE; 1-DEOXY-D-XYLULOSE-5-PHOSPHATE SYNTHASE; BIOSYNTHESIS; EXPRESSION; CLONING; GENE; PATHWAY; ELICITATION; PROTEIN; CDNA;

D O I：

10.1007/s11738-012-0940-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.

引用

页码：1421 / 1433

页数：13

共 40 条

[1] Enhanced flux through the methylerythritol 4-phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase [J].

Carretero-Paulet, Lorenzo ;

Cairo, Albert ;

Botella-Pavia, Patricia ;

Besumbes, Oscar ;

Campos, Narciso ;

Boronat, Albert ;

Rodriguez-Concepcion, Manuel .

PLANT MOLECULAR BIOLOGY, 2006, 62 (4-5) :683-695

[2] BIOCHEMISTRY AND MOLECULAR-BIOLOGY OF THE ISOPRENOID BIOSYNTHETIC-PATHWAY IN PLANTS [J].

CHAPPELL, J .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1995, 46 :521-547

[3] Application of non-aqueous micellar electrokinetic chromatography to the analysis of active components in radix Salviae miltiorrhizae and its medicinal preparations [J].

Chen, AJ ;

Li, CH ;

Gao, WH ;

Hu, ZD ;

Chen, XG .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 37 (04) :811-816

[4] Candidate genes involved in tanshinone biosynthesis in hairy roots of Salvia miltiorrhiza revealed by cDNA microarray [J].

Cui, Guanghong ;

Huang, Luqi ;

Tang, Xiaojing ;

Zhao, Jingxue .

MOLECULAR BIOLOGY REPORTS, 2011, 38 (04) :2471-2478

[5] Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq [J].

Engprasert S. ;

Taura F. ;

Kawamukai M. ;

Shoyama Y. .

BMC Plant Biology, 4 (1)

[6] 1-deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants [J].

Estévez, JM ;

Cantero, A ;

Reindl, A ;

Reichler, S ;

León, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (25) :22901-22909

[7] Knock-down of the MEP pathway isogene 1-deoxy-D-xylulose 5-phosphate synthase 2 inhibits formation of arbuscular mycorrhiza-induced apocarotenoids, and abolishes normal expression of mycorrhiza-specific plant marker genes [J].

Floss, Daniela S. ;

Hause, Bettina ;

Lange, Peter R. ;

Kuester, Helge ;

Strack, Dieter ;

Walter, Michael H. .

PLANT JOURNAL, 2008, 56 (01) :86-100

[8]

Gao Wei, 2008, Yaoxue Xuebao, V43, P766

[9] A Functional Genomics Approach to Tanshinone Biosynthesis Provides Stereochemical Insights [J].

Gao, Wei ;

Hillwig, Matthew L. ;

Huang, Luqi ;

Cui, Guanghong ;

Wang, Xueyong ;

Kong, Jianqiang ;

Yang, Bin ;

Peters, Reuben J. .

ORGANIC LETTERS, 2009, 11 (22) :5170-5173

[10] Induction and potentiation of diterpenoid tanshinone accumulation in Salvia miltiorrhiza hairy roots by β-aminobutyric acid [J].

Ge, XC ;

Wu, JY .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 68 (02) :183-188

← 1 2 3 4 →