Analysis of genetic diversity in cowpea (Vigna unguiculata L.Walp.) cultivars with random amplified polymorphic DNA markers

被引:12
|
作者
Malviya, Neha [1 ]
Sarangi, B. K. [2 ]
Yadav, Manoj Kumar [3 ]
Yadav, Dinesh [1 ]
机构
[1] DDU Gorakhpur Univ, Dept Biotechnol, Gorakhpur 273009, Uttar Pradesh, India
[2] Natl Environm Engn Res Inst, Environm Biotechnol Div, Nagpur 440020, Maharashtra, India
[3] SVP Univ Agr & Technol, Dept Biotechnol, Coll Agr, Meerut 250110, Uttar Pradesh, India
关键词
Cowpea; RAPD marker; Genetic diversity; Dendrogram; Cultivars; Polymorphism; RFLP; RAPD; GERMPLASM; AFLP; WILD; PCR;
D O I
10.1007/s00606-011-0545-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The genetic diversity among ten Indian cultivars of cowpea was analyzed using 18 sets of RAPD markers. A total of 181 bands with an average of 15 bands per primer were obtained. Out of 181 bands, 148 showed polymorphism (81.7%). The variation in genetic diversity among these cultivars ranged from 0.1742 to 0.4054. Cluster analysis based on Jaccard's similarity coefficient using UPGMA with high bootstrap values revealed two distinct clusters I and II comprised of two and seven cultivars, respectively. Cluster II was further differentiated into various subclusters. Cultivar IC-9883 was found to be unique based on its altogether distinct position in the dendrogram and two-dimensional space projections.
引用
收藏
页码:523 / 526
页数:4
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