DNA aptamers selection for carcinoembryonic antigen (CEA)

被引：17

作者：

Abreu de Melo, Mariane Izabella ^{[1
]}

Correa, Cristiane Rodrigues ^{[1
]}

Cunha, Pricila da Silva ^{[1
]}

de Goes, Alfredo Miranda ^{[2
]}

Gomes, Dawidson Assis ^{[2
]}

Ribeiro de Andrade, Antero Silva ^{[1
]}

机构：

[1] Comissao Nacl Energia Nucl, Ctr Desenvolvimento Tecnol Nucl, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG, Brazil

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2020年 / 30卷 / 15期

关键词：

COLON-CANCER CELLS; BREAST-CANCER; RNA APTAMER; LABEL-FREE; APTASENSOR; MOLECULES; DNAZYME;

D O I：

10.1016/j.bmcl.2020.127278

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Carcinoembryonic antigen (CEA) is a glycoprotein antigen generally used for diagnosis, prognosis and treatment monitoring of several types of tumors, including colorectal cancer. Nucleic acid aptamers are DNA or RNA oligonucleotides capable of binding with high specificity and affinity to a molecular target. The aim of this study was to obtain aptamers specific to CEA for use as radiopharmaceuticals in colorectal cancer diagnosis. Five aptamers were selected through the Systematic Evolution of Ligands by EXponencial Enrichment (SELEX) and tested using T84 (CEA+) and Hela (CEA−) cells. Apta 3 and Apta 5 showed the best results presenting high specificity and affinity for T84 cells, with dissociation constants (Kd) of 60.4 ± 5.7 nM and 37.8 ± 5.8 nM, respectively. These results indicate that Apta 3 and Apta 5 are promising candidates for identifying tumor cells that overexpress CEA. © 2020 Elsevier Ltd

引用

收藏

页数：6

相关论文

共 38 条

[1] Nucleic acid aptamers in diagnosis of colorectal cancer [J].

Ahmadyousefi, Yaghoub ;

Malih, Sara ;

Mirzaee, Younes ;

Saidijam, Massoud .

BIOCHIMIE, 2019, 156 :1-11

[2]

Barros ALB, 2019, COMPREHENSIVE GUIDE, V1, P71

[3] Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in cancer progression and metastasis [J].

Beauchemin, Nicole ;

Arabzadeh, Azadeh .

CANCER AND METASTASIS REVIEWS, 2013, 32 (3-4) :643-671

[4] New Prostate Cancer Targets for Diagnosis, Imaging, and Therapy: Focus on Prostate-Specific Membrane Antigen [J].

Cimadamore, Alessia ;

Cheng, Monica ;

Santoni, Matteo ;

Lopez-Beltran, Antonio ;

Battelli, Nicola ;

Massari, Francesco ;

Galosi, Andrea B. ;

Scarpelli, Marina ;

Montironi, Rodolfo .

FRONTIERS IN ONCOLOGY, 2018, 8

[5] Aptamers directly radiolabeled with technetium-99m as a potential agent capable of identifying carcinoembryonic antigen (CEA) in tumor cells T84 [J].

Correa, Cristiane Rodrigues ;

Branco de Barros, Andre Luis ;

Ferreira, Carolina de Aguiar ;

de Goes, Alfredo Miranda ;

Cardoso, Valbert Nascimento ;

Ribeiro de Andrade, Antero Silva .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2014, 24 (08) :1998-2001

[6] Anti-MUC1 aptamers: radiolabelling with 99mTc and biodistribution in MCF-7 tumour-bearing mice [J].

Da Pieve, Chiara ;

Perkins, Alan C. ;

Missailidis, Sotiris .

NUCLEAR MEDICINE AND BIOLOGY, 2009, 36 (06) :703-710

[7] A label-free aptasensor for carcinoembryonic antigen detection using three-way junction structure and ATMND as a fluorescent probe [J].

Danesh, Noor Mohammad ;

Yazdian-Robati, Rezvan ;

Ramezani, Mohammad ;

Alibolandi, Mona ;

Abnous, Khalil ;

Taghdisi, Seyed Mohammad .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 256 :408-412

[8] Anti-MUC1 nano-aptamers for triple-negative breast cancer imaging by single-photon emission computed tomography in inducted animals: initial considerations [J].

do Carmo, Fagner Santos ;

Ricci-Junior, Eduardo ;

Cerqueira-Coutinho, Cristal ;

Albernaz, Marta de Souza ;

Bernardes, Emerson Soares ;

Missailidis, Sotiris ;

Santos-Oliveira, Ralph .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2017, 12 :53-60

[9] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[10] Aptamers as radiopharmaceuticals for nuclear imaging and therapy [J].

Gijs, Marlies ;

Aerts, An ;

Impens, Nathalie ;

Baatout, Sarah ;

Luxen, Andre .

NUCLEAR MEDICINE AND BIOLOGY, 2016, 43 (04) :253-271

← 1 2 3 4 →