Comparative performance evaluation of hepatitis C virus genotyping based on the 5′ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
被引:15
作者:
Nakatani, Sueli M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
LACEN PR, Lab Cent Estado Parana, Sao Jose Dos Pinhais, Parana, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Nakatani, Sueli M.
[1
,2
]
Santos, Carlos A.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Genomas, Sao Paulo, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Santos, Carlos A.
[3
]
Riediger, Irina N.
论文数: 0引用数: 0
h-index: 0
机构:
LACEN PR, Lab Cent Estado Parana, Sao Jose Dos Pinhais, Parana, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Riediger, Irina N.
[2
]
Krieger, Marco A.
论文数: 0引用数: 0
h-index: 0
机构:
ICC FioCruz, Curitiba, Parana, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Krieger, Marco A.
[4
]
Duarte, Cesar A. B.
论文数: 0引用数: 0
h-index: 0
机构:
ICC FioCruz, Curitiba, Parana, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Duarte, Cesar A. B.
[4
]
Debur, Maria do Carmo
论文数: 0引用数: 0
h-index: 0
机构:
LACEN PR, Lab Cent Estado Parana, Sao Jose Dos Pinhais, Parana, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Debur, Maria do Carmo
[2
]
Carrilho, Flair J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Carrilho, Flair J.
[1
]
Ono, Suzane K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, BrazilUniv Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
Ono, Suzane K.
[1
]
机构:
[1] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Sao Paulo, Brazil
[2] LACEN PR, Lab Cent Estado Parana, Sao Jose Dos Pinhais, Parana, Brazil
Background: Genotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region. Methods: Plasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis. Results: Comparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B. Conclusions: Sequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains.