Immunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells

被引：27

作者：

Liu, Zhidong ^{[1
]}

Liu, Zhifang ^{[1
]}

Li, Laihao ^{[2
]}

Zhang, Junjie ^{[3
]}

Zhao, Qiancheng ^{[4
]}

Lin, Na ^{[1
]}

Zhong, Wenzhu ^{[5
]}

Jiang, Mei ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, East China Sea Fishery Res Inst, 300 Jungong Rd, Shanghai 200090, Peoples R China

[2] Chinese Acad Fishery Sci, South China Sea Fishery Res Inst, Guangzhou, Peoples R China

[3] Jiangsu Ocean Univ, Coll Food Sci & Engn, Lianyungang, Peoples R China

[4] Dalian Ocean Univ, Coll Food Sci & Engn, Dalian, Peoples R China

[5] Chinese Acad Fishery Sci, Fishery Machinery & Instrument Res Inst, Shanghai, Peoples R China

来源：

FOOD SCIENCE & NUTRITION | 2022年 / 10卷 / 04期

关键词：

immunomodulatory activity; macrophage; polysaccharide; Sinonovacula constricta; STRUCTURAL-CHARACTERIZATION; IMMUNOSTIMULATORY POLYSACCHARIDES; NEUTRAL POLYSACCHARIDE; SIGNALING PATHWAYS; ANTIOXIDANT; PURIFICATION; IDENTIFICATION; MAPK; ACID; L;

D O I：

10.1002/fsn3.2735

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP-1-1) in RAW264.7 cells, SCP-1-1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP-1-1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF-alpha, IL-6, and IL-1 beta) secretion of RAW264.7 cell in a dose-dependent manner. Western blot analysis results demonstrated that SCP-1-1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF-kappa beta and P13K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta.

引用

页码：1093 / 1102

页数：10

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