Differential microscopy for fluorescence-detected nonlinear absorption linear anisotropy based on a staggered two-beam femtosecond Yb:KGW oscillator

被引：51

作者：

Sandkuijl, Daaf ^{[1
,2
]}

Cisek, Richard ^{[1
,2
]}

Major, Arkady ^{[3
]}

Barzda, Virginijus ^{[1
,2
]}

机构：

[1] Univ Toronto, Dept Chem & Phys Sci, Dept Phys, Mississauga, ON L5L 1C6, Canada

[2] Univ Toronto, Inst Opt Sci, Mississauga, ON L5L 1C6, Canada

[3] Univ Manitoba, Dept Elect & Comp Engn, Winnipeg, MB R3T 5V6, Canada

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 03期

基金：

加拿大自然科学与工程研究理事会; 加拿大创新基金会;

关键词：

D O I：

10.1364/BOE.1.000895

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new laser system and nonlinear microscope, designed for differential nonlinear microscopy. The microscope features time-correlated single photon counting of multiphoton fluorescence generated by an alternating pulse-train of orthogonally polarized pulses. The generated nonlinear signal is separated using home-built electronics. Results are presented on fluorescence-detected nonlinear absorption linear anisotropy (FDNALA) of chloroplasts in Asparagus Sprengerii Regel and of Congo Red-stained cellulose. (C) 2010 Optical Society of America

引用

页码：895 / 901

页数：7

共 12 条

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