Direct electrochemistry of hemoglobin based on nano-composite film of gold nanopaticles and poly (diallyldimethylammonium chloride) functionalized graphene

被引：69
作者：
Feng, Qingliang [1 ,2 ]
Liu, Kunping [3 ]
Fu, Jiajia [1 ,2 ]
Zhang, Yuzhen [1 ,2 ]
Zheng, Zhixiang [1 ,2 ]
Wang, Chunming [1 ,2 ]
Du, Yongling [1 ,2 ]
Ye, Weichun [1 ,2 ]
机构：
[1] Lanzhou Univ, Sch Chem & Chem Engn, Lanzhou 730000, Peoples R China
[2] Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
[3] Chengdu Univ, Fac Biotechnol Ind, Chengdu 610106, Peoples R China
来源：
ELECTROCHIMICA ACTA | 2012年 / 60卷
基金：
中国国家自然科学基金;
关键词：
Hemoglobin; AuNPs/graphene; Poly (diallyldimethylammonium chloride); Hydrogen peroxide; Direct electrochemistry; HYDROGEN-PEROXIDE; BIOSENSOR; ELECTROCATALYSIS; IMMOBILIZATION; NANOPARTICLES; FABRICATION; HB;
D O I：
10.1016/j.electacta.2011.11.048
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
The poly (diallyldimethylammonium chloride) (PDDA) functionalized graphene nanosheets (PDDA-G) were prepared and gold nano-particles (AuNPs) were assembled on the surface of PDDA-G film through electrostatic interaction to construct the biocompatible interface. The resulting AuNPs/PDDA-G nanocomposite film exhibited enhanced capability for hemoglobin (Hb) immobilization and realized its direct electrochemistry. The surface concentration (Gamma*) of electroactive Hb on the surface of GCE was calculated to be 3.85 x 10(-9) mol/cm(2). Moreover, the AuNPs/PDDA-G based sensor showed prominent electrocatalytic activity for the detection of H2O2 with a wide linear range from 6 mu M to 1010 mu M and a low detection limit of 0.39 mu M at 3 sigma. Meanwhile, the apparent Michaelis-Menten constant (K-m) was 0.51 mM. This biosensor exhibited high sensitivity and fast responses, which may provide a novel way for fabrication of other graphene based sensors and broaden the application of graphene in biosensing. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：304 / 308
页数：5
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