Atmospheric plasma enhances wettability and cell spreading on dental implant metals

被引:201
作者
Duske, Kathrin [1 ,3 ]
Koban, Ina [1 ]
Kindel, Eckhard [2 ]
Schroeder, Karsten [2 ]
Nebe, Barbara [3 ]
Holtfreter, Birte [1 ]
Jablonowski, Lukasz [1 ]
Weltmann, Klaus D. [2 ]
Kocher, Thomas [1 ]
机构
[1] Ernst Moritz Arndt Univ Greifswald, Dept Restorat Dent Periodontol & Endodontol, Sch Dent, Unit Periodontol, D-17475 Greifswald, Germany
[2] Leibniz Inst Plasma Sci & Technol eV, Greifswald, Germany
[3] Univ Rostock, Dept Cell Biol, Med Res Ctr, Fac Med, Rostock, Germany
关键词
biomaterials; dental implants; implant surface; osteoblasts MG-63; plasma technology; surface preparation; OSTEOBLAST-LIKE CELLS; SURFACE MODIFICATION; PRESSURE PLASMA; POLYMER SURFACES; IN-VITRO; TITANIUM; ROUGHNESS; ADHESION; PROLIFERATION; FIBROBLAST;
D O I
10.1111/j.1600-051X.2012.01853.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives: Treatment regimens, which predictably support re-osseointegration of implants with peri-implantitis, are needed. Increased wettability may be an important factor for re-osseointegration. In this study, a cold atmospheric pressure gas-discharge plasma was applied to reduce water contact angles on titanium discs with different surface topography and to improve the spreading of osteoblastic cells. Material and Methods: An argon plasma jet with different oxygen admixtures was used to treat titanium discs with different topologies, i. e. machined, SLA r, SLActive r, diamond bur-treated or Airflow r -treated. Water contact angles were measured before and after plasma treatment. The spreading behaviour of human osteoblastic cells was investigated. Results: Contact angle of titanium discs (baseline values: 68 -117) were significantly reduced close to 0 irrespective of surface topography after the application of argon plasma with 1.0% oxygen admixture for 60 s or 120 s. The cell size of osteoblastic cells grown on argon-oxygen-plasma-treated titanium discs was significantly larger than on non-treated surfaces (p < 0.001) irrespective of surface topography. Conclusions: Plasma treatment reduced contact angle and supported spreading of osteoblastic cells. The application of cold plasma may be supportive in the treatment of peri-implant lesions and may improve the process of re-osseointegration.
引用
收藏
页码:400 / 407
页数:8
相关论文
共 38 条
[1]  
[Anonymous], 2011, Stata statistical software: Release 12
[2]  
Aronsson BO, 1997, J BIOMED MATER RES, V35, P49
[3]   Enhanced bone apposition to a chemically modified SLA titanium surface [J].
Buser, D ;
Broggini, N ;
Wieland, M ;
Schenk, RK ;
Denzer, AJ ;
Cochran, DL ;
Hoffmann, B ;
Lussi, A ;
Steinemann, SG .
JOURNAL OF DENTAL RESEARCH, 2004, 83 (07) :529-533
[4]   Surgical treatment of peri-implantitis [J].
Claffey, Noel ;
Clarke, Emily ;
Polyzois, Ioannis ;
Renvert, Stefan .
JOURNAL OF CLINICAL PERIODONTOLOGY, 2008, 35 :316-332
[5]   Protein adhesion and cell response on atmospheric pressure dielectric barrier discharge-modified polymer surfaces [J].
D'Sa, Raechelle A. ;
Burke, George A. ;
Meenan, Brian J. .
ACTA BIOMATERIALIA, 2010, 6 (07) :2609-2620
[6]   Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds [J].
Ermolaeva, Svetlana A. ;
Varfolomeev, Alexander F. ;
Chernukha, Marina Yu. ;
Yurov, Dmitry S. ;
Vasiliev, Mikhail M. ;
Kaminskaya, Anastasya A. ;
Moisenovich, Mikhail M. ;
Romanova, Julia M. ;
Murashev, Arcady N. ;
Selezneva, Irina I. ;
Shimizu, Tetsuji ;
Sysolyatina, Elena V. ;
Shaginyan, Igor A. ;
Petrov, Oleg F. ;
Mayevsky, Evgeny I. ;
Fortov, Vladimir E. ;
Morfill, Gregor E. ;
Naroditsky, Boris S. ;
Gintsburg, Alexander L. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (01) :75-83
[7]   Non-thermal atmospheric pressure discharges for surface modification [J].
Foest, R ;
Kindel, E ;
Ohl, A ;
Stieber, M ;
Weltmann, KD .
PLASMA PHYSICS AND CONTROLLED FUSION, 2005, 47 :B525-B536
[8]  
Fridman A., 2008, PLASMA CHEM PLASMA P, P259
[9]   Comparison of direct and indirect effects of non-thermal atmospheric-pressure plasma on bacteria [J].
Fridman, Gregory ;
Brooks, Ari D. ;
Balasubramanian, Manjula ;
Fridman, Alexander ;
Gutsol, Alexander ;
Vasilets, Victor N. ;
Ayan, Halim ;
Friedman, Gary .
PLASMA PROCESSES AND POLYMERS, 2007, 4 (04) :370-375
[10]   Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment [J].
Garcia, Jorge Lopez ;
Asadinezhad, Ahmad ;
Pachernik, Jiri ;
Lehocky, Marian ;
Junkar, Ita ;
Humpolicek, Petr ;
Saha, Petr ;
Valasek, Pavel .
MOLECULES, 2010, 15 (04) :2845-2856