Simple HPLC determination of colistin in medicated feeds by pre-column derivatization and fluorescence detection

被引：19

作者：

Cancho-Grande, B ^{[1
]}

Rodríguez-Comesana, M ^{[1
]}

Simal-Gándara, J ^{[1
]}

机构：

[1] Univ Vigo, Fac Food Sci & Technol, Nutr & Bromatol Grp, Analyt & Food Chem Dept, Orense 32004, Spain

来源：

CHROMATOGRAPHIA | 2001年 / 54卷 / 7-8期

关键词：

column liquid chromatography; pre-column derivatization; fluorescence detection; colistin; medicated feeds;

D O I：

10.1007/BF02491203

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive HPLC method was developed for the determination of colistin antibiotic in feeds employing pre-column derivatization and fluorescence detection. Extraction of colistin in feeds was by sonication and shaking with 0.1 M HCL Pre-column derivatization was with phthaldialdehyde and 2-mercaptoethanol (ME) in borate buffer (pH 10.5) to obtain a fluorescent derivative. Elution of the derivative onto an Ultracarb 5 mum ODS column was by using acetonitrile - ultrapure water (75:25). Detection was by spectrofluorimetry at 340 nm (excitation wavelength) and 440 nm (emission wavelength). Total elution time was < 20 min. The applicability of the validated method was tested by analyzing commercial medicated feeds without any interference from the matrix.

引用

页码：481 / 484

页数：4

共 9 条

[1]

*ACS, 1980, ANAL CHEM, V14, P83

[2]

BOPPANA V, 1991, J CHROMATOGR, V584, P319

[3] Hyphenated liquid chromatographic method for the determination of colistin residues in bovine tissues [J].