Detection of immunomagnetically captured Escherichia coli O157:H7 by antibody-conjugated alkaline phosphatase

被引：12

作者：

Tu, SI ^{[1
]}

Patterson, D ^{[1
]}

Briggs, C ^{[1
]}

Irwin, P ^{[1
]}

Yu, L ^{[1
]}

机构：

[1] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2001年 / 26卷 / 06期

关键词：

immunomagnetic capture; bacterial detection; antibody-conjugated alkaline phosphatase; bacterial detection method;

D O I：

10.1038/sj.jim.7000141

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid and sensitive detection process for Escherichia coli O157:H7 was developed using alkaline phosphatase (APase)-labeled anti-E. coli O157 antibodies to tag the targeted bacteria. Immunomagnetic beads or antibody-labeled streptavidin-coated magnetic beads were then used to capture the APase-tagged E. coli. Immunomagnetically captured bacteria were washed and distributed into microplates or optical cuvettes. The enzyme-catalyzed hydrolysis of p-nitro-phenol phosphate in alkaline solutions was then followed. Less than 1000 cfu/ml of E. coli O157:H7 could be detected. This approach was applied to detect the bacteria artificially spiked in beef hamburgers. Less than 1 cfu/g of E. coli O157:H7 produced a significant response after cultural enrichment for 4-6 h at 37 degreesC.

引用

页码：345 / 349

页数：5

共 21 条

[1] DETECTION OF SALMONELLA SPP IN OYSTERS BY PCR [J].