How Do We Use Multicolor Flow Cytometry to Detect Minimal Residual Disease in Acute Myeloid Leukemia?

被引：45

作者：

Xu, Jie ^{[1
]}

Jorgensen, Jeffrey L. ^{[1
]}

Wang, Sa A. ^{[1
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, 1515 Holcombe Blvd,Unit 72, Houston, TX 77030 USA

来源：

CLINICS IN LABORATORY MEDICINE | 2017年 / 37卷 / 04期

关键词：

Multicolor flow cytometry; Acute myeloid leukemia; Minimal residual disease; HEMATOPOIETIC-CELL TRANSPLANTATION; TREATMENT RESPONSE; COMPLETE REMISSION; CLONAL EVOLUTION; OLDER PATIENTS; ONCOLOGY-GROUP; RELAPSE RISK; DIAGNOSIS; SURVIVAL; THERAPY;

D O I：

10.1016/j.cll.2017.07.004

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Multicolor flow cytometry (MFC), combined with molecular and cytogenetic studies, is the most common method for detecting minimal residual disease (MRD) in acute myeloid leukemia (AML). Studies have shown that a positive MFC MRD study after induction and/or consolidation, or before allogeneic hematopoietic stem cell transplantation, correlates with risk of relapse and inferior survival. However, there is little information on technical and analytical details. This article shares the authors' experience using "MFC for AML MRD detection, including antibody panel design, data analysis, and interpretation. It summarizes diagnostic pearls and pitfalls and provides practical information for pathologists or hematologists.

引用

页码：787 / +

页数：17

共 50 条

[1] The use of flow cytometry to detect minimal residual disease in acute leukemia
Campana, D
CoustanSmith, E
EUROPEAN JOURNAL OF HISTOCHEMISTRY, 1996, 40 : 39 - 42
[2] Minimal residual disease detection in cryopreserved ovarian tissue by multicolor flow cytometry in acute myeloid leukemia
Zver, Tristan
Alvergnas-Vieille, Magalie
Garnache-Ottou, Francine
Ferrand, Christophe
Roux, Christophe
Amiot, Clotilde
HAEMATOLOGICA, 2014, 99 (12)
[3] Flow cytometry in acute myeloid leukemia and detection of minimal residual disease
Cai, Qihui
Lan, Haiqiang
Yi, Deng
Xian, Bojun
Zidan, Luo
Li, Jianqiao
Liao, Zhaohong
CLINICA CHIMICA ACTA, 2025, 564
[4] A refined approach to detect and measure minimal residual disease in childhood acute myeloid leukemia by flow cytometry
Capolunghi, Federica
Capponi, Claudia
De Stefani, Barbara
Luciani, Matteo
Locatelli, Franco
Muraca, Maurizio
Carsetti, Rita
AMERICAN JOURNAL OF HEMATOLOGY, 2014, 89 (03) : 343 - 344
[5] Multidimensional flow cytometry for detection of minimal residual disease in acute myeloid leukemia
Venditti, A
Maurillo, L
Buccisano, F
Tamburini, A
Del Poeta, G
Del Principe, MI
Panetta, P
Consalvo, MI
Mazzone, C
Tendas, A
Trawinska, M
Forte, V
Amadori, S
LEUKEMIA & LYMPHOMA, 2003, 44 (03) : 445 - 450
[6] Minimal Residual Disease Monitoring by Flow Cytometry in Pediatric Acute Myeloid Leukemia
Rubnitz, J.
ANNALS OF HEMATOLOGY, 2013, 92 : S55 - S55
[7] AN EFFICIENT AND SIMPLE MULTIPARAMETER FLOW CYTOMETRY PROTOCOL TO DETECT MINIMAL RESIDUAL DISEASE (MRD) FOR ACUTE MYELOID LEUKEMIA (AML)
Li, Ke
Wang, Hui
BONE MARROW TRANSPLANTATION, 2023, 58 (SUPP1) : 356 - 357
[8] Impact of pretransplant minimal residual disease detection by flow cytometry in acute myeloid leukemia
Perez, E.
Lopez-Godino, O.
Cabrero, M.
Labrador, J.
Alonso, S.
Perez, J. J.
Lopez-Corral, L.
Vazquez, L.
Gonzalez, M.
Hernadez, J. M.
Puig, N.
Diez-Campelo, M.
Martin, A.
Mateos, M. V.
Paiva, B.
del Canizo, M. C.
San Miguel, J.
Caballero, M. D.
Vidriales, M. B.
BONE MARROW TRANSPLANTATION, 2015, 50 : S238 - S239
[9] The Role of Multiparameter Flow Cytometry for Detection of Minimal Residual Disease in Acute Myeloid Leukemia
Al-Mawali, Adhra
Gillis, David
Lewis, Ian
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2009, 131 (01) : 16 - 26
[10] Defining minimal residual disease by flow cytometry in acute myeloid leukemia: the HARMONIZE initiative
Buecklein, V. L.
Krause, S.
Oelschlaegel, U.
Koehnke, T.
Schnorfeil, F.
Voelkl, S.
Rieger, M. A.
Berg, T.
Hoffmann, J.
Brendel, C.
Hiddemann, W.
Subklewe, M.
von Bonin, M.
ONCOLOGY RESEARCH AND TREATMENT, 2017, 40 : 43 - 43

← 1 2 3 4 5 →