Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings

被引：13

作者：

Milky, Bridget ^{[1
,2
]}

Zabolocki, Michael ^{[1
,2
]}

Al-Bataineh, Sameer A. ^{[3
]}

van den Hurk, Mark ^{[1
]}

Greenberg, Zarina ^{[1
]}

Turner, Lucy ^{[1
]}

Mazzachi, Paris ^{[1
,2
]}

Williams, Amber ^{[1
,2
]}

Illeperuma, Imanthi ^{[1
]}

Adams, Robert ^{[1
,2
]}

Stringer, Brett W. ^{[1
,2
]}

Ormsby, Rebecca ^{[2
]}

Poonnoose, Santosh ^{[2
]}

Smith, Louise E. ^{[3
,5
,6
]}

Krasowska, Marta ^{[5
]}

Whittle, Jason D. ^{[4
,6
]}

Simula, Antonio ^{[3
,6
]}

Bardy, Cedric ^{[1
,2
]}

机构：

[1] South Australian Hlth & Med Res Inst SAHMRI, Lab Human Neurophysiol & Genet, Adelaide, SA, Australia

[2] Flinders Univ S Australia, Flinders Hlth & Med Res Inst FHMRI, Coll Med & Publ Hlth, Adelaide, SA, Australia

[3] TekCyte Ltd, Adelaide, SA, Australia

[4] Univ South Australia STEM, Mawson Lakes Campus, Mawson Lakes, SA, Australia

[5] Univ South Australia STEM, Future Ind Inst, Mawson Lakes Campus, Mawson Lakes, SA, Australia

[6] Cooperat Res Ctr Cell Therapy Mfg CTM CRC, Adelaide, SA, Australia

来源：

STEM CELL REPORTS | 2022年 / 17卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

PLURIPOTENT STEM-CELLS; NERVE GROWTH-FACTOR; EXTRACELLULAR-MATRIX; SHEAR FORCE; NEURONS; SURFACE; ADHESION; 1,3-DIAMINOPROPANE; DIFFERENTIATION; POLYPYRROLE;

D O I：

10.1016/j.stemcr.2022.01.013

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Advances in cellular reprogramming have radically increased the use of patient-derived cells for neurological research in vitro. However, adherence of human neurons on tissue cultureware is unreliable over the extended periods required for electrophysiological maturation. Adherence issues are particularly prominent for transferable glass coverslips, hindering imaging and electrophysiological assays. Here, we assessed thin-film plasma polymer treatments, polymeric factors, and extracellular matrix coatings for extending the adherence of human neuronal cultures on glass. We find that positive-charged, amine-based plasma polymers improve the adherence of a range of human brain cells. Diaminopropane (DAP) treatment with laminin-based coating optimally supports long-term maturation of fundamental ion channel properties and synaptic activity of human neurons. As proof of concept, we demonstrated that DAP-treated glass is ideal for live imaging, patch-clamping, and optogenetics. A DAP-treated glass surface reduces the technical variability of human neuronal models and enhances electrophysiological maturation, allowing more reliable discoveries of treatments for neurological and psychiatric disorders.

引用

页码：489 / 506

页数：18

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