Activity of Matrix Metalloproteinases in Bovine versus Human Dentine

被引:19
作者
Kato, M. T. [1 ]
Hannas, A. R. [1 ]
Leite, A. L. [1 ]
Bolanho, A. [1 ]
Zarella, B. L. [1 ]
Santos, J. [2 ]
Carrilho, M. [3 ]
Tjaderhane, L. [4 ,5 ]
Buzalaf, M. A. R. [1 ]
机构
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP, Brazil
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Area, Dept Restorat Dent, Piracicaba, Brazil
[3] Univ Bandeirante Sao Paulo, Sch Dent, GEO UNIBAN, Sao Paulo, Brazil
[4] Univ Oulu, Inst Dent, Oulu, Finland
[5] Oulu Univ Hosp, Oulu, Finland
基金
巴西圣保罗研究基金会;
关键词
Bovine tooth; Dentine; Gelatinase; Human tooth; Matrix metalloproteinases; ENAMEL; EROSION; COLLAGENASE; INHIBITION; ABRASION;
D O I
10.1159/000330525
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Metalloproteinases (MMPs) have been implicated with metabolism of collagen in physiological and pathological processes in human dentine. As bovine teeth have been used as a substitute for human teeth in laboratory analysis, this study evaluated the activity of MMP-2 and -9 in bovine versus human dentine. Bovine and human dentine fragments, from crowns and roots, were powderized. Protein extraction was performed by two protocols: a neutral extraction with guanidine-HCl/EDTA (pH 7.4) and an acidic extraction with citric acid (pH 2.3). Gelatinolytic activities of extracts were revealed by zymography. MMP-2 and -9 were detected in crown and root dentine from bovine and human teeth. Total activities of MMP-2 were 11.4 +/- 2.2, 14.6 +/- 2.0, 9.7 +/- 1.2 and 12.4 +/- 0.9 ng/ml for bovine root, human root, bovine crown and human crown dentine, respectively. Corresponding activities for MMP-9 were 14.9 +/- 2.0, 15.3 +/- 1.3, 15.4 +/- 1.3 and 15.5 +/- 1.3 ng/ml, respectively. Bovine dentine was found to be a reliable substrate for studies involving the activity of MMP-2 and -9. Copyright (C) 2011 S. Karger AG, Basel
引用
收藏
页码:429 / 434
页数:6
相关论文
共 29 条
[1]   Cloning, characterization, and expression analysis of mouse enamelysin [J].
Caterina, J ;
Shi, J ;
Sun, X ;
Qian, Q ;
Yamada, S ;
Liu, Y ;
Krakora, S ;
Bartlett, JD ;
Yamada, Y ;
Engler, JA ;
Birkedal-Hansen, H ;
Simmer, JP .
JOURNAL OF DENTAL RESEARCH, 2000, 79 (09) :1697-1703
[2]   THE PRESENCE AND POSSIBLE FUNCTIONS OF THE MATRIX METALLOPROTEINASE COLLAGENASE ACTIVATOR PROTEIN IN DEVELOPING ENAMEL MATRIX [J].
DENBESTEN, PK ;
HEFFERNAN, LM ;
TREADWELL, BV ;
AWBREY, BJ .
BIOCHEMICAL JOURNAL, 1989, 264 (03) :917-920
[3]   Radiodensity and hardness of enamel and dentin of human and bovine teeth, varying bovine teeth age [J].
Fonseca, R. B. ;
Haiter-Neto, F. ;
Carlo, H. L. ;
Soares, C. J. ;
Sinhoreti, M. A. C. ;
Puppin-Rontani, R. M. ;
Correr-Sobrinho, L. .
ARCHIVES OF ORAL BIOLOGY, 2008, 53 (11) :1023-1029
[4]   Effect of erosive pH cycling on different restorative materials and on enamel restored with these materials [J].
Francisconi, L. F. ;
Honorio, H. M. ;
Rios, D. ;
Magalhaes, A. C. ;
Machado, M. A. A. M. ;
Buzalaf, M. A. R. .
OPERATIVE DENTISTRY, 2008, 33 (02) :203-208
[5]   The role of matrix metalloproteinases in the oral environment [J].
Hannas, Angelica R. ;
Pereira, Jose C. ;
Granjeiro, Jose M. ;
Tjaderhane, Leo .
ACTA ODONTOLOGICA SCANDINAVICA, 2007, 65 (01) :1-13
[6]   Caries progression and inhibition in human and bovine root dentine in situ [J].
Hara, AT ;
Queiroz, CS ;
Leme, AF ;
Serra, MC ;
Cury, JA .
CARIES RESEARCH, 2003, 37 (05) :339-344
[7]   Effect of Iron on Matrix Metalloproteinase Inhibition and on the Prevention of Dentine Erosion [J].
Kato, M. T. ;
Leite, A. L. ;
Hannas, A. R. ;
Oliveira, R. C. ;
Pereira, J. C. ;
Tjaderhane, L. ;
Buzalaf, M. A. R. .
CARIES RESEARCH, 2010, 44 (03) :309-316
[8]   Gels Containing MMP Inhibitors Prevent Dental Erosion in situ [J].
Kato, M. T. ;
Leite, A. L. ;
Hannas, A. R. ;
Buzalaf, M. A. R. .
JOURNAL OF DENTAL RESEARCH, 2010, 89 (05) :468-472
[9]   PROTECTIVE EFFECT OF GREEN TEA ON DENTIN EROSION AND ABRASION [J].
Kato, Melissa Thiemi ;
Magalhaes, Ana Carolina ;
Rios, Daniela ;
Hannas, Angelica Reis ;
Attin, Thomas ;
Rabelo Buzalaf, Marilia Afonso .
JOURNAL OF APPLIED ORAL SCIENCE, 2009, 17 (06) :560-564
[10]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265