High LET Radiation Enhances Nocodazole Induced Cell Death in HeLa Cells through Mitotic Catastrophe and Apoptosis

被引:6
|
作者
Li, Ping [1 ,2 ,3 ]
Zhou, Libin [1 ]
Dai, Zhongying [1 ,2 ,3 ]
Jin, Xiaodong [1 ,3 ]
Liu, Xinguo [1 ,3 ]
Matsumoto, Yoshitaka [4 ]
Furusawa, Yoshiya [4 ]
Li, Qiang [1 ,3 ]
机构
[1] Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
[3] Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
[4] Natl Inst Radiol Sci, Heavy Ion Radiobiol Res Grp, Chiba 2638555, Japan
基金
中国国家自然科学基金;
关键词
HeLa cells; Nocodazole; High LET radiation; Mitotic catastrophe; Apoptosis; SPINDLE-ASSEMBLY CHECKPOINT; DNA-DAMAGE; ARREST; P53; CYTOKINESIS; MECHANISMS;
D O I
10.1269/jrr.10186
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
To understand how human tumor cells respond to the combined treatment with nocodazole and high LET radiation, alterations in cell cycle, mitotic disturbances and cell death were investigated in the present study. Human cervix carcinoma HeLa cells were exposed to nocodazole for 18 h immediately followed by high LET iron ion irradiation and displayed a sequence of events leading to DNA damages, mitotic aberrations, interphase restitution and endocycle as well as cell death. A prolonged mitotic arrest more than 10 h was observed following nocodazole exposure, no matter the irradiation was present or not. The occurrence of mitotic slippage following the mitotic arrest was only drug-dependent and the irradiation did not accelerate it. The amount of polyploidy cells was increased following mitotic slippage. No detectable G(2) or G(1) arrest was observed in cells upon the combined treatment and the cells reentered the cell cycle still harboring unrepaired cellular damages. This premature entry caused an increase of multipolar mitotic spindles and amplification of centrosomes, which gave rise to lagging chromosomal material, failure of cytokinesis and polyploidization. These mitotic disturbances and their outcomes confirmed the incidence of mitotic catastrophe and delayed apoptotic features displayed by TUNEL method after the combined treatment. These results suggest that the addition of high-LET iron ion irradiation to nocodazole enhanced mitotic catastrophe and delayed apoptosis in HeLa cells. These might be important cell death mechanisms involved in tumor cells in response to the treatment of antimitotic drug combined with high LET radiation.
引用
收藏
页码:481 / 489
页数:9
相关论文
共 50 条
  • [1] AURORA-A KINASE INHIBITION ENHANCES THE CYTOSINE ARABINOSIDE-INDUCED CELL DEATH IN LEUKEMIA CELLS THROUGH APOPTOSIS AND MITOTIC CATASTROPHE
    Cheong, J. W.
    Min, Y. H.
    Jung, H. I.
    Eom, J. I.
    Kim, S. J.
    Jeung, H.
    Kim, J. S.
    HAEMATOLOGICA-THE HEMATOLOGY JOURNAL, 2010, 95 : 687 - 688
  • [2] Aurora-A kinase inhibition enhances the cytosine arabinoside-induced cell death in leukemia cells through apoptosis and mitotic catastrophe
    Cheong, June-Won
    Jung, Haeng-Im
    Eom, Ju In
    Kim, Soo Jung
    Jeung, Hoi-Kyung
    Min, Yoo Hong
    CANCER LETTERS, 2010, 297 (02) : 171 - 181
  • [3] Casticin induces leukemic cell death through apoptosis and mitotic catastrophe
    Shen, Jia-Kun
    Du, Hua-ping
    Yang, Min
    Wang, Yun-Gui
    Jin, Jie
    ANNALS OF HEMATOLOGY, 2009, 88 (08) : 743 - 752
  • [4] Casticin induces leukemic cell death through apoptosis and mitotic catastrophe
    Jia-Kun Shen
    Hua-ping Du
    Min Yang
    Yun-Gui Wang
    Jie Jin
    Annals of Hematology, 2009, 88 : 743 - 752
  • [5] Two distinct modes of cell death induced by doxorubicin: apoptosis and cell death through mitotic catastrophe accompanied by senescence-like phenotype
    Eom, YW
    Kim, MA
    Park, SS
    Goo, MJ
    Kwon, HJ
    Sohn, S
    Kim, WH
    Yoon, G
    Choi, KS
    ONCOGENE, 2005, 24 (30) : 4765 - 4777
  • [6] Two distinct modes of cell death induced by doxorubicin: apoptosis and cell death through mitotic catastrophe accompanied by senescence-like phenotype
    Young-Woo Eom
    Mi Ae Kim
    Seok Soon Park
    Mi Jin Goo
    Hyuk Jae Kwon
    Seonghyang Sohn
    Wook-Hwan Kim
    Gyesoon Yoon
    Kyeong Sook Choi
    Oncogene, 2005, 24 : 4765 - 4777
  • [7] Reducing the radiation-induced G(2) delay causes HeLa cells to undergo apoptosis instead of mitotic death
    Bernhard, EJ
    Muschel, RJ
    Bakanauskas, VJ
    McKenna, WG
    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1996, 69 (05) : 575 - 584
  • [8] Depletion of Paraspeckle Protein 1 Enhances Methyl Methanesulfonate-Induced Apoptosis through Mitotic Catastrophe
    Gao, Xiangjing
    Zhang, Guanglin
    Shan, Shigang
    Shang, Yunlong
    Chi, Linfeng
    Li, Hongjuan
    Cao, Yifei
    Zhu, Xinqiang
    Zhang, Meibian
    Yang, Jun
    PLOS ONE, 2016, 11 (01):
  • [9] Bcl-xL blocks high dose doxorubicin-induced apoptosis but not low dose doxorubicin-induced cell death through mitotic catastrophe
    Park, Seok Soon
    Kim, Mi Ae
    Eom, Young-Woo
    Choi, Kyeong Sook
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 363 (04) : 1044 - 1049
  • [10] Docetaxel induces cell death through mitotic catastrophe in human breast cancer cells
    Morse, DL
    Gray, H
    Payne, CM
    Gillies, RJ
    MOLECULAR CANCER THERAPEUTICS, 2005, 4 (10) : 1495 - 1504