Wnt signaling modulates macrophage polarization and is regulated by biomaterial surface properties

被引:120
作者
Abaricia, Jefferson O. [1 ]
Shah, Arth H. [1 ]
Chaubal, Manotri [1 ]
Hotchkiss, Kelly M. [1 ]
Olivares-Navarrete, Rene [1 ]
机构
[1] Virginia Commonwealth Univ, Coll Engn, Dept Biomed Engn, Richmond, VA USA
基金
美国国家卫生研究院;
关键词
Macrophage; Wnt signaling; Microstructured titanium; DIFFERENTIATION; CELLS; REPAIR; INFLAMMATION; ACTIVATION; PHENOTYPE; PATHWAY; STRESS;
D O I
10.1016/j.biomaterials.2020.119920
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Macrophages are among the first cells to interact with biomaterials and ultimately determine their integrative fate. Biomaterial surface characteristics like roughness and hydrophilicity can activate macrophages to an anti-inflammatory phenotype. Wnt signaling, a key cell proliferation and differentiation pathway, has been associated with dysregulated macrophage activity in disease. However, the role Wnt signaling plays in macrophage activation and response to biomaterials is unknown. The aim of this study was to characterize the regulation of Wnt signaling in macrophages during classical pro- and anti-inflammatory polarization and in their response to smooth, rough, and rough-hydrophilic titanium (Ti) surfaces. Peri-implant Wnt signaling in macrophage-ablated (MaFIA) mice instrumented with intramedullary Ti rods was significantly attenuated compared to untreated controls. Wnt ligand mRNA were upregulated in a surface modification-dependent manner in macrophages isolated from the surface of Ti implanted in C57B1/6 mice. In vitro, Wnt mRNAs were regulated in primary murine bone-marrow-derived macrophages cultured on Ti in a surface modification-dependent manner. When macrophageal Wnt secretion was inhibited, macrophage sensitivity to both physical and biological stimuli was abrogated. Loss of macrophage-derived Wnts also impaired recruitment of mesenchymal stem cells and T-cells to Ti implants in vivo. Finally, inhibition of integrin signaling decreased surface-dependent upregulation of Writ genes. These results suggest that Wnt signaling regulates macrophage response to biomaterials and that macrophages are an important source of Wnt ligands during inflammation and healing.
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页数:17
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