Lactobacillus paracasei K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells

被引：5

作者：

Chondrou, P. ^{[1
]}

Karapetsas, A. ^{[1
]}

Kiousi, D. E. ^{[1
]}

Tsela, D. ^{[1
]}

Tiptiri-Kourpeti, A. ^{[1
]}

Anestopoulos, I ^{[1
]}

Kotsianidis, I ^{[2
]}

Bezirtzoglou, E. ^{[3
]}

Pappas, A. ^{[1
]}

Galanis, A. ^{[1
]}

机构：

[1] Democritus Univ Thrace, Dept Mol Biol & Genet, Fac Hlth Sci, Alexandroupolis 68100, Greece

[2] Democritus Univ Thrace, Dept Hematol, Med Sch, Fac Hlth Sci, Alexandroupolis 68100, Greece

[3] Democritus Univ Thrace, Fac Agr & Forestry, Dept Agr Dev, Orestiada 68200, Greece

来源：

BENEFICIAL MICROBES | 2018年 / 9卷 / 06期

关键词：

Lactobacillus; probiotics; adhesion; proliferation; apoptosis; CASEI ATCC 393; LACTIC-ACID BACTERIA; RHAMNOSUS GG; IN-VITRO; PROBIOTICS; SURVIVAL; MODULATION; PREVENTION; DIARRHEA;

D O I：

10.3920/BM2017.0183

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.

引用

页码：975 / 983

页数：9

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