Excited-State Intermolecular Proton Transfer of Firefly Luciferin IV. Temperature and pH Dependence

被引：29

作者：

Erez, Yuval ^{[1
]}

Presiado, Itay ^{[1
]}

Gepshtein, Rinat ^{[1
]}

Huppert, Dan ^{[1
]}

机构：

[1] Tel Aviv Univ, Raymond & Beverly Sackler Fac Exact Sci, Sch Chem, IL-69978 Tel Aviv, Israel

来源：

JOURNAL OF PHYSICAL CHEMISTRY A | 2011年 / 115卷 / 09期

基金：

以色列科学基金会;

关键词：

GEMINATE RECOMBINATION; QUANTUM YIELD; BIOLUMINESCENCE; OXYLUCIFERIN; FLUORESCENCE; DIFFUSION; PYRANINE; ORIGIN; ICE;

D O I：

10.1021/jp110889v

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Time-resolved emission as well as steady-state UV-vis techniques were employed to study the photoprotolytic processes that D-luciferin, the natural substrate of the firefly luciferase, undergoes in both acidic aqueous solutions and ice. The emission spectrum of D-luciferin in a 20 mM HCl aqueous solution or higher has an additional emission band at 590 nm red-shifted with respect to the strongest emission band positioned at 530 nm of the deprotonated NRO-* form in a pH-neutral aqueous solution. We attribute this emission band to the zwitterion form designated as +HNRO-. The time-resolved emission signals show that the NRO-* emission band at 530 nm and the zwitterion emission band at 590 are strongly quenched by a recombination process with a proton in an acidic solution and in ice. In ice, the quenching rate is 10 times faster than in the liquid state:. We attribute the fast quenching rate to the high value of the proton diffusion constant in ice.

引用

页码：1617 / 1626

页数：10

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