Pulsed DC Electric Field-Induced Differentiation of Cortical Neural Precursor Cells

被引：40

作者：

Chang, Hui-Fang ^{[1
]}

Lee, Ying-Shan ^{[2
]}

Tang, Tang K. ^{[2
]}

Cheng, Ji-Yen ^{[1
,3
,4
,5
]}

机构：

[1] Acad Sinica, Res Ctr Appl Sci, Taipei, Taiwan

[2] Acad Sinica, Inst Biomed Sci, Taipei, Taiwan

[3] Natl Yang Ming Univ, Inst Biophoton, Taipei, Taiwan

[4] Natl Yang Ming Univ, Biophoton & Mol Imaging Res Ctr BMIRC, Taipei, Taiwan

[5] Natl Taiwan Ocean Univ, Dept Mech & Mechantron Engn, Keelung, Taiwan

来源：

PLOS ONE | 2016年 / 11卷 / 06期

关键词：

NEURITE GROWTH; STEM-CELLS; PROGENITOR CELLS; RETINOIC ACID; CHICK-EMBRYO; SPINAL-CORD; IN-VIVO; SOX2; MIGRATION; NEURONS;

D O I：

10.1371/journal.pone.0158133

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the differentiation of neural stem and progenitor cells solely induced by direct current (DC) pulses stimulation. Neural stem and progenitor cells in the adult mammalian brain are promising candidates for the development of therapeutic neuroregeneration strategies. The differentiation of neural stem and progenitor cells depends on various in vivo environmental factors, such as nerve growth factor and endogenous EF. In this study, we demonstrated that the morphologic and phenotypic changes of mouse neural stem and progenitor cells (mNPCs) could be induced solely by exposure to square-wave DC pulses (magnitude 300 mV/mm at frequency of 100-Hz). The DC pulse stimulation was conducted for 48 h, and the morphologic changes of mNPCs were monitored continuously. The length of primary processes and the amount of branching significantly increased after stimulation by DC pulses for 48 h. After DC pulse treatment, the mNPCs differentiated into neurons, astrocytes, and oligodendrocytes simultaneously in stem cell maintenance medium. Our results suggest that simple DC pulse treatment could control the fate of NPCs. With further studies, DC pulses may be applied to manipulate NPC differentiation and may be used for the development of therapeutic strategies that employ NPCs to treat nervous system disorders.

引用

页数：16